枯草芽孢杆菌转化后的基因表达。
Gene expression after transformation of Bacillus subtilis.
作者信息
Chasin L A, Magasanik B
出版信息
J Bacteriol. 1970 Jun;102(3):661-5. doi: 10.1128/jb.102.3.661-665.1970.
Abstract
Sensitive assays of histidase activity were used to follow the production of this enzyme as directed by a gene newly introduced into cells of Bacillus subtilis by transformation. Histidase activity can be detected in histidase-negative recipient cells within 1 hr after the addition of deoxyribonucleic acid extracted from histidase-positive donors. Enzyme production continues for one to two additional hours and then ceases. Histidase production in the transformed cells is fully sensitive to catabolite repression. Catabolite repression is rapidly established after transformation of recipient cells that are resistant to this form of regulation.
摘要
采用对组氨酸酶活性敏感的检测方法，来追踪由通过转化新导入枯草芽孢杆菌细胞的一个基因所指导的该酶的产生情况。在添加从组氨酸酶阳性供体中提取的脱氧核糖核酸后1小时内，可在组氨酸酶阴性受体细胞中检测到组氨酸酶活性。酶的产生会持续额外的一到两个小时，然后停止。转化细胞中组氨酸酶的产生对分解代谢物阻遏完全敏感。在对这种调控形式有抗性的受体细胞转化后，分解代谢物阻遏会迅速建立。
相似文献
1
Gene expression after transformation of Bacillus subtilis.枯草芽孢杆菌转化后的基因表达。
J Bacteriol. 1970 Jun;102(3):661-5. doi: 10.1128/jb.102.3.661-665.1970.
2
Modulation of Bacillus subtilis catabolite repression by transition state regulatory protein AbrB.过渡态调节蛋白AbrB对枯草芽孢杆菌分解代谢物阻遏的调控
J Bacteriol. 1994 Apr;176(7):1903-12. doi: 10.1128/jb.176.7.1903-1912.1994.
3
Induction and repression of the histidine-degrading enzymes of Bacillus subtilis.枯草芽孢杆菌组氨酸降解酶的诱导与抑制
J Biol Chem. 1968 Oct 10;243(19):5165-78.
4
Comparative studies on induction of sporulation and synthesis of inducible enzymes in Bacillus subtilis.枯草芽孢杆菌中孢子形成诱导及诱导酶合成的比较研究。
J Bacteriol. 1974 Dec;120(3):1102-8. doi: 10.1128/jb.120.3.1102-1108.1974.
5
2-Ketoglutarate and the regulation of aconitase and histidase formation in Bacillus subtilis.2-酮戊二酸与枯草芽孢杆菌中乌头酸酶和组氨酸酶形成的调节
J Bacteriol. 1984 Apr;158(1):379-82. doi: 10.1128/jb.158.1.379-382.1984.
6
Regulation of histidine and proline degradation enzymes by amino acid availability in Bacillus subtilis.枯草芽孢杆菌中氨基酸可用性对组氨酸和脯氨酸降解酶的调控
J Bacteriol. 1990 Sep;172(9):4758-65. doi: 10.1128/jb.172.9.4758-4765.1990.
7
Cloning and nucleotide sequences of histidase and regulatory genes in the Bacillus subtilis hut operon and positive regulation of the operon.枯草芽孢杆菌组氨酸酶操纵子中组氨酸酶及调控基因的克隆、核苷酸序列分析以及该操纵子的正调控
J Bacteriol. 1988 Jul;170(7):3199-205. doi: 10.1128/jb.170.7.3199-3205.1988.
8
Relationship between transformation frequency and gene function in the histidine degrading enzymes of Bacillus subtilis.
Biochem Biophys Res Commun. 1969 Mar 31;34(6):777-83. doi: 10.1016/0006-291x(69)90247-2.
9
Activation of the Bacillus subtilis hut operon at the onset of stationary growth phase in nutrient sporulation medium results primarily from the relief of amino acid repression of histidine transport.在营养芽孢形成培养基中，枯草芽孢杆菌组氨酸操纵子在稳定生长期开始时的激活主要源于组氨酸转运的氨基酸阻遏作用的解除。
J Bacteriol. 1993 Jul;175(14):4282-9. doi: 10.1128/jb.175.14.4282-4289.1993.
10
Transformation of Bacillus subtilis in alpha-amylase productivity by deoxyribonucleic acid from B. subtilis var. amylosacchariticus.利用解淀粉芽孢杆菌变种的脱氧核糖核酸对枯草芽孢杆菌进行α-淀粉酶生产力的转化。
J Bacteriol. 1974 Dec;120(3):1144-50. doi: 10.1128/jb.120.3.1144-1150.1974.
引用本文的文献
1
Macromolecular synthesis in Bacillus subtilis during development of the competent state.枯草芽孢杆菌感受态发育过程中的大分子合成
J Bacteriol. 1971 Nov;108(2):668-79. doi: 10.1128/jb.108.2.668-679.1971.
2
Mechanism of resistance to 5-azacytidine in Bacillus subtilis. I. Isolation and some properties of mutants resistant to 5-azacytidine and 5-aza-2'-deoxycytidine.
Folia Microbiol (Praha). 1972;17(6):517-21. doi: 10.1007/BF02872738.
3
Expression of an excision repair gene in transformation of Bacillus subtilis.切除修复基因在枯草芽孢杆菌转化中的表达
Mol Gen Genet. 1977 Jun 8;153(2):129-33. doi: 10.1007/BF00264727.
本文引用的文献
1
Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA.枯草芽孢杆菌 DNA 两条互补链的转录活性。
Science. 1967 Aug 18;157(3790):817-9. doi: 10.1126/science.157.3790.817.
2
REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌转化的要求。
J Bacteriol. 1961 May;81(5):741-6. doi: 10.1128/jb.81.5.741-746.1961.
3
PROPERTIES OF NEWLY INTRODUCED TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.枯草芽孢杆菌中新引入的转化脱氧核糖核酸的特性
J Bacteriol. 1965 Aug;90(2):343-6. doi: 10.1128/jb.90.2.343-346.1965.
4
FATE OF TRANSFORMING DEOXYRIBONUCLEIC ACID IN BACILLUS SUBTILIS.枯草芽孢杆菌中转化脱氧核糖核酸的命运
J Bacteriol. 1965 May;89(5):1250-5. doi: 10.1128/jb.89.5.1250-1255.1965.
5
[BIOCHEMISTRY OF SPOROGENESIS IN B. SUBTILIS].[枯草芽孢杆菌孢子形成的生物化学]
Bull Soc Chim Biol (Paris). 1964;46:443-81.
6
PENICILLIN RESISTANCE OF COMPETENT CELLS IN DEOXYRIBONUCLEIC ACID TRANSFORMATION OF BACILLUS SUBTILIS.枯草芽孢杆菌脱氧核糖核酸转化中感受态细胞的青霉素抗性
J Bacteriol. 1964 Apr;87(4):867-75. doi: 10.1128/jb.87.4.867-875.1964.
7
TRANSFORMATION OF BACILLUS SUBTILIS TO MOTILITY AND PROTOTROPHY: MICROMANIPULATIVE ISOLATION OF BACTERIA OF TRANSFORMED PHENOTYPE.枯草芽孢杆菌向运动性和原养型的转化：转化表型细菌的显微操作分离
J Bacteriol. 1963 Oct;86(4):797-804. doi: 10.1128/jb.86.4.797-804.1963.
8
BIOSYNTHETIC LATENCY IN EARLY STAGES OF DEOXYRIBONUCLEIC ACIDTRANSFORMATION IN BACILLUS SUBTILIS.枯草芽孢杆菌中脱氧核糖核酸转化早期阶段的生物合成潜伏期
J Bacteriol. 1963 Oct;86(4):785-96. doi: 10.1128/jb.86.4.785-796.1963.
9
Formation of amylomaltase after genetic transformation of pneumococcus.肺炎球菌基因转化后支链淀粉酶的形成。
Biochim Biophys Acta. 1960 Dec 4;45:155-63. doi: 10.1016/0006-3002(60)91436-0.
10
Genetic control of repression of alkaline phosphatase in E. coli.大肠杆菌中碱性磷酸酶阻遏的遗传控制
J Mol Biol. 1961 Aug;3:425-38. doi: 10.1016/s0022-2836(61)80055-7.
Zotero 插件