Cervini R, Rocchi M, DiDonato S, Finocchiaro G
Istituto Nazionale Neurologico, Divisione di Biochimica e Genetica, Milan, Italy.
Neurosci Lett. 1991 Nov 11;132(2):191-4. doi: 10.1016/0304-3940(91)90299-9.
A DNA fragment of 219 bp was obtained by polymerase chain reaction (PCR) on human genomic DNA using two oligonucleotide mixtures derived from peptide sequences of human placenta choline acetyltransferase (ChAT) and from partially conserved amino acid sequences of rat, porcine and Drosophila ChAT. Sequence homology with porcine ChAT demonstrated that this fragment is part of the human ChAT gene. This gene was assigned to chromosome 10 by hybridization of the 219 bp DNA probe with DNA from human-hamster somatic cell hybrids, and to region 10q11.2-10qter by PCR experiments.
利用从人胎盘胆碱乙酰转移酶（ChAT）的肽序列以及大鼠、猪和果蝇ChAT的部分保守氨基酸序列衍生而来的两种寡核苷酸混合物，通过聚合酶链反应（PCR）在人基因组DNA上获得了一个219 bp的DNA片段。与猪ChAT的序列同源性表明，该片段是人ChAT基因的一部分。通过将219 bp DNA探针与人-仓鼠体细胞杂种的DNA杂交，该基因被定位于10号染色体，通过PCR实验定位于10q11.2 - 10qter区域。
