Zuki A B Z, Hafeez Y M, Loqman M Y, Noordin M M, Norimah Y
Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia.
Anat Histol Embryol. 2007 Oct;36(5):349-56. doi: 10.1111/j.1439-0264.2007.00772.x.
This study investigates the effect of preservation methods on the performance of bovine parietal pericardium grafts in a rat model. Mid-ventral full thickness abdominal wall defects of 3 x 2.5 cm in size were created in 90 male Sprague-Dawley rats (300-400 g), which were divided into three groups of 30 rats each. The abdominal defects of group one and two were repaired with lyophilized and glycerolized bovine pericardium grafts, while the defects of group three were repaired with expanded polytetrafluoroethylene (ePTFE) Mycro Mesh as a positive control. Another group of 30 rats underwent sham operation and was used for comparison as negative control. Each group of rats (n = 30) was divided into five subgroups (n = 6) and killed at 1, 3, 6, 9 and 18 weeks post-surgery for gross and morphological evaluations. The rats tolerated the surgical procedure well with a total mortality of 0.05%. No serious post-operative clinical complications or signs of rejection were encountered. Adhesions between the grafts and the underlying visceral organs observed in the study were mostly results of post-surgical complications. Glycerol preservation delayed degradation and replacement of the grafts, whereas lyophilization caused early resorption and replacement of the grafts. The glycerolized grafts were replaced with thick dense fibrous tissue, and the lyophilized grafts were replaced with thin loose fibrous tissue. The healing characteristic of the bovine pericardium grafts was similar to those of the sham-operated group, and quite different from those of the ePTFE Mycro Mesh. The outcome of the present study confirmed the superiority of glycerolized bovine pericardium grafts over its lyophilized counter part.
本研究在大鼠模型中调查了保存方法对牛心包移植物性能的影响。在90只雄性Sprague-Dawley大鼠（300 - 400克）身上制造了大小为3×2.5厘米的腹正中全层腹壁缺损，将其分为三组，每组30只大鼠。第一组和第二组的腹部缺损用冻干和甘油化的牛心包移植物修复，而第三组的缺损用膨体聚四氟乙烯（ePTFE）Mycro Mesh修复作为阳性对照。另一组30只大鼠接受假手术并用作阴性对照进行比较。每组大鼠（n = 30）分为五个亚组（n = 6），并在术后1、3、6、9和18周处死以进行大体和形态学评估。大鼠对手术过程耐受性良好，总死亡率为0.05%。未遇到严重的术后临床并发症或排斥迹象。研究中观察到的移植物与下方内脏器官之间的粘连大多是手术并发症的结果。甘油保存延迟了移植物的降解和替代，而冻干则导致移植物早期吸收和替代。甘油化移植物被厚而致密的纤维组织替代，冻干移植物被薄而疏松的纤维组织替代。牛心包移植物的愈合特征与假手术组相似，与ePTFE Mycro Mesh组有很大不同。本研究结果证实了甘油化牛心包移植物优于其冻干对应物。
