Faculty of Veterinary Medicine, University Putra Malaysia, 43400, Serdang, Selangor, Darul Ehsan, Malaysia.
J Mater Sci Mater Med. 2010 May;21(5):1721-30. doi: 10.1007/s10856-010-4007-7. Epub 2010 Feb 5.
The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3 x 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of the first group were repaired with myoblast-seeded-bovine tunica vaginalis whereas the second group repaired with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at 7th, 14th, and 30th days of post-implantation from each group and the explanted specimens were subjected to macroscopic and microscopic analysis. In every case, seeded scaffolds have better deposition of newly formed collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibers were only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine tunica vaginalis can be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal muscle tissue.
本研究旨在构建骨骼肌组织用于修复腹壁缺损。肌母细胞接种到支架上，并在体外培养 5 天。在 18 只雄性新西兰白兔中创建了全层腹壁缺损（3x4cm），并将其随机分为两组。第一组的缺损用肌母细胞接种的牛鞘膜修复，而第二组用未接种的牛鞘膜修复作为对照。每组分别有 3 只动物在植入后第 7、14 和 30 天被处死，取出的标本进行宏观和微观分析。在每种情况下，接种支架的新形成的胶原蛋白沉积和新血管生成均优于对照组。有趣的是，只有在细胞接种组中才检测到多核肌管和肌纤维。本研究表明，肌母细胞接种的牛鞘膜可用作有效支架，用于修复严重和大的腹壁缺损，并再生骨骼肌组织。
