Mizuno T, McKinnon A, Ichihara N, Amasaki T, Asari M, Nishita T, Oishi M, Soeta S, Amasaki H
Australia-Japan Wildlife Conservation and Education Foundation, 148 Haven Road, Pullenvale, Qld 4069, Australia.
Anat Histol Embryol. 2009 Nov;38(6):449-54. doi: 10.1111/j.1439-0264.2009.00971.x. Epub 2009 Sep 28.
While the mandibular glands usually consist of only mucous acinar cells or a combination of mucous and serous cells in other species of mammals, those of koalas were serous glands. Rabbit mono-specific polyclonal anti-canine CA-I, II, III or VI antiserum showed cross-reactivity against corresponding koala carbonic anhydrase (CA) isozymes. Although immunohistochemical reactions to CA-I, II and VI in ductal cells were moderate to strong in the tested salivary glands, no reaction or only slight reactions were observed against CA-III. In the sublingual glands, moderate immunohistochemical reactions to CA-I, II and VI were also evident in serous acinar cells and serous demilunes. However, no reactions to the tested isozymes were observed in mucous acinar cells in these glands. With the exception of the histological structure of the mandibular glands, histological features and the distributional profile of CA isozymes of the salivary glands in koalas are relatively close to results obtained from horses.
虽然在其他种类的哺乳动物中，下颌腺通常仅由黏液腺泡细胞组成，或由黏液细胞和浆液细胞混合组成，但考拉的下颌腺是浆液腺。兔单特异性抗犬CA-I、II、III或VI多克隆抗血清与相应的考拉碳酸酐酶（CA）同工酶呈现交叉反应。尽管在所测试的唾液腺中，导管细胞对CA-I、II和VI的免疫组化反应为中度至强阳性，但对CA-III未观察到反应或仅观察到轻微反应。在舌下腺中，浆液性腺泡细胞和浆液半月对CA-I、II和VI的中度免疫组化反应也很明显。然而，在这些腺体的黏液性腺泡细胞中未观察到对所测试同工酶的反应。除下颌腺的组织结构外，考拉唾液腺的组织学特征和CA同工酶的分布情况与马的结果相对接近。
