Hisbergues M, Magi M, Rigaux P, Steuve J, Garcia L, Goudercourt D, Pot B, Pestel J, Jacquet A
Laboratory of Lactic Acid Bacteria and Mucosal Immunity, Pasteur Institute of Lille, Institute of Biology of Lille, Lille Cedex, France.
Clin Exp Allergy. 2007 Sep;37(9):1286-95. doi: 10.1111/j.1365-2222.2007.02792.x.
Lactic acid bacteria (LAB) were reported to reduce some allergic manifestations in mice and humans but their impact on the aeroallergen-dependent immune mechanisms is still debated.
The potential capacities of Lactobacillus plantarum NCIMB8826 to reduce the allergic response induced by Der p 1, the major house dust mite allergen of Dermatophagoides pteronyssinus, were evaluated in vivo and in vitro. Methods First, the effect of the intranasal co-administration of LAB and purified Der p 1 allergen before a sensitization protocol was evaluated. The allergen-specific antibody and cellular responses as well as airway inflammation were measured. Second, the impact of LAB on the cytokine profile of spleens cells from Der p 1-sensitized mice was assessed. Third, upon stimulation with LAB, the levels of cytokine produced by dendritic cells derived from the bone marrow (BMDCs) of wild-type, Toll-like receptor 2 (TLR2)-, TLR4- and MyD88-KO mice were compared. Results The co-application of L. plantarum and Der p 1 induced a T-helper type 1 (Th1)-biased allergen-specific IgG response, the absence of specific IgE response and favoured the production of INF-gamma upon allergen re-stimulation. Moreover, the previous LAB administration reduced the development of bronchoalveolar lavage eosinophilia usually induced by aerosol exposure. Additionally, the studied LAB strain was shown to modify in vitro the cytokine level produced by Der p 1-sensitized spleen cells mainly towards a Th1 profile. Finally, L. plantarum stimulated high IL-12 and moderate IL-10 production in mouse BMDCs notably through the TLR2-, MyD88-dependent and TLR4-independent pathway.
In vivo co-administration of probiotic LAB with Der p 1 might prevent the development of the mite allergic response. The probiotic L. plantarum was shown to display in vitro therapeutic potentials for the treatment of allergy and to trigger the immune system by a TLR2- and MyD88-dependent signalling pathway.
据报道，乳酸菌（LAB）可减轻小鼠和人类的一些过敏表现，但其对气传变应原依赖性免疫机制的影响仍存在争议。
在体内和体外评估植物乳杆菌NCIMB8826降低由Der p 1（粉尘螨主要屋尘螨变应原）诱导的过敏反应的潜在能力。方法：首先，评估在致敏方案前鼻内联合给予LAB和纯化的Der p 1变应原的效果。检测变应原特异性抗体和细胞反应以及气道炎症。其次，评估LAB对Der p 1致敏小鼠脾细胞细胞因子谱的影响。第三，用LAB刺激后，比较野生型、Toll样受体2（TLR2）、TLR4和MyD88基因敲除小鼠骨髓来源的树突状细胞（BMDCs）产生的细胞因子水平。结果：植物乳杆菌与Der p 1联合应用诱导了以T辅助1型（Th1）为主的变应原特异性IgG反应，无特异性IgE反应，并在变应原再次刺激时促进了INF-γ的产生。此外，先前给予LAB减少了通常由气溶胶暴露诱导的支气管肺泡灌洗嗜酸性粒细胞增多的发生。另外，所研究的LAB菌株在体外显示可改变Der p 1致敏脾细胞产生的细胞因子水平，主要趋向于Th1谱。最后，植物乳杆菌显著通过TLR2、MyD88依赖性和TLR4非依赖性途径刺激小鼠BMDCs产生高IL-12和中等水平的IL-10。
益生菌LAB与Der p 1在体内联合应用可能预防螨过敏反应的发生。益生菌植物乳杆菌在体外显示出治疗过敏的潜在潜力，并通过TLR2和MyD88依赖性信号通路触发免疫系统。
