Yousuf Seema, Atif Fahim, Hoda Nasrul, Ahmad Muzamil, Saleem Sofiyan, Ishrat Tauheed, Khan M Badruzzaman, Ahmad Abdullah Shafique, Islam Fakhrul
Department of Surgery, Section of Neurosurgery, University of Nebraska Medical Center, Omaha, NE, USA.
Basic Clin Pharmacol Toxicol. 2007 Oct;101(4):246-53. doi: 10.1111/j.1742-7843.2007.00110.x.
Majun Baladar (MB), a traditional herbal formulation of the Unani system of medicine, was studied for its efficacy against cerebral ischaemia-induced oxidative damage in hippocampus and associated neurobehavioural deficits. Adult male Wistar rats were divided into four groups. The first group was sham, the second group was ischaemic (MCAO: middle cerebral artery occluded) and the third group was a MB pre-treated ischaemic group (MCAO + MB). The fourth group was given MB (1.05 g/kg) orally for 15 days as a drug control. The middle cerebral artery was occluded for 2 hr and reperfused for 22 hr in the ischaemic as well as the drug pre-treated group. The activity of the various enzymatic antioxidants like glutathione peroxidase, glutathione reductase, glutathione S-transferase and non-enzymatic antioxidants, glutathione along with levels of lipid peroxidation were evaluated. Cerebral ischaemic rats showed elevated level of lipid peroxidation and decreased levels of various antioxidants significantly over sham values. As a result of MB pre-treatment, the level of lipid peroxidation was found to be significantly depleted as compared to the ischaemic group. Furthermore, depleted levels of glutathione and the activity of glutathione peroxidase, glutathione S-transferase and glutathione reductase were restored significantly in MB treated group. Majun Baladar exhibited a significant improvement in neurobehavioural activities in the drug pre-treated animals as compared to the ischaemic group as evidenced by the grip strength test, Rota-Rod and video path analysis. The results of the present study provide baseline information regarding the neuroprotective efficacy of MB and also open a window for a potent therapeutic use of this traditional herbal Unani medicine.
Majun Baladar（MB）是尤纳尼医学体系中的一种传统草药配方，针对其对大脑缺血诱导的海马体氧化损伤及相关神经行为缺陷的疗效进行了研究。成年雄性Wistar大鼠被分为四组。第一组为假手术组，第二组为缺血组（大脑中动脉闭塞：MCAO），第三组为MB预处理缺血组（MCAO + MB）。第四组作为药物对照组，口服MB（1.05 g/kg），持续15天。在缺血组和药物预处理组中，大脑中动脉闭塞2小时，再灌注22小时。评估了各种酶促抗氧化剂如谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽S -转移酶的活性以及非酶促抗氧化剂谷胱甘肽，同时还评估了脂质过氧化水平。大脑缺血大鼠与假手术组相比，脂质过氧化水平显著升高，各种抗氧化剂水平显著降低。由于MB预处理，与缺血组相比，脂质过氧化水平显著降低。此外，可以看到MB治疗组中谷胱甘肽水平的降低以及谷胱甘肽过氧化物酶、谷胱甘肽S -转移酶和谷胱甘肽还原酶的活性显著恢复。与缺血组相比，在药物预处理动物中，通过握力测试、转棒试验和视频路径分析证明，Majun Baladar在神经行为活动方面有显著改善。本研究结果提供了关于MB神经保护疗效的基线信息，也为这种传统尤纳尼草药的有效治疗应用打开了一扇窗。
