Siqueira I R, Fochesatto C, Torres I L S, da Silva A L, Nunes D S, Elisabetsky E, Netto C A
Departamento de Farmacologia, Universidade Federal do Rio Grande do Sul, Rua Sarmento Leite, 500, sala 202, 90050-170 Porto Alegre RS, Brazil.
Phytomedicine. 2007 Nov;14(11):763-9. doi: 10.1016/j.phymed.2006.12.007. Epub 2007 Apr 12.
Ptychopetalum olacoides (PO) roots are used by Amazonian peoples to prepare traditional remedies for treating various central nervous system conditions in which free radicals are likely to be implicated. Following the identification of PO ethanol extract (POEE) free-radical scavenging properties in vitro, the aim of this study was to verify the in vivo antioxidant effect of POEE. Aging mice (14 months) were treated (i.p.) with saline, DMSO (20%) or POEE (100mg/kg body wt.), and the hippocampi, cerebral cortex, striata, hypothalamus and cerebellum dissected out 60 min later to measure antioxidant enzyme activities, free-radical production and damage to macromolecules. POEE administration reduced free-radical production in the hypothalamus, lead to significant decrease in lipid peroxidation in the cerebral cortex, striatum and hypothalamus, as well as in the carbonyl content in cerebellum and striatum. In terms of antioxidant enzymes, catalase activity was increased in the cortex, striatum, cerebellum and hippocampus, while glutathione peroxidase activity was increased in the hippocampus. This study suggests that POEE contains compounds able to improve the cellular antioxidant network efficacy in the brain, ultimately reducing the damage caused by oxidative stress.
亚马逊地区的人们使用卵叶瘤药树（Ptychopetalum olacoides，简称PO）的根来制备传统药物，用于治疗各种可能与自由基有关的中枢神经系统疾病。在体外鉴定出PO乙醇提取物（POEE）具有自由基清除特性后，本研究的目的是验证POEE在体内的抗氧化作用。对14个月大的衰老小鼠腹腔注射生理盐水、二甲基亚砜（20%）或POEE（100mg/kg体重），60分钟后取出海马体、大脑皮层、纹状体、下丘脑和小脑，测量抗氧化酶活性、自由基产生以及对大分子的损伤。给予POEE可减少下丘脑的自由基产生，导致大脑皮层、纹状体和下丘脑的脂质过氧化显著降低，以及小脑和纹状体的羰基含量降低。就抗氧化酶而言，大脑皮层、纹状体、小脑和海马体中的过氧化氢酶活性增加，而海马体中的谷胱甘肽过氧化物酶活性增加。本研究表明，POEE含有能够提高大脑细胞抗氧化网络功效的化合物，最终减少氧化应激造成的损伤。
