Tommila Miretta, Jokinen Jutta, Wilson Timothy, Forsback A-P, Saukko Pekka, Penttinen Risto, Ekholm Erika
Departments of Medical Biochemistry and Molecular Biology, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland.
Acta Biomater. 2008 Mar;4(2):354-61. doi: 10.1016/j.actbio.2007.07.005. Epub 2007 Jul 28.
Granulation tissue was induced in hydroxyapatite-coated cellulose sponges with subcutaneous implantation in rats. A massive inflammatory reaction with an intense foreign body reaction and an increased invasion of fibrovascular tissue was observed by days 1-3 post-operation, whereas tissue growth into the uncoated control implants was much slower and took place mainly on their surfaces. The foreign body reaction in apatite-coated sponges declined after post-operative day 14, and no obvious differences were seen between the two cellulose sponges from 1 month up to 1 year after implantation. The apatite-coated implants attracted macrophages and fibroblasts, and favored angiogenesis. The excessive connective tissue formation was histologically normal, synthesized the major extracellular matrix molecules in a normal ratio and did not seem to disturb the animals in any way. These results warrant further investigations on clinical applicability of hydroxyapatite-coated cellulose sponges, when fast proliferation of connective tissue is desirable.
通过在大鼠皮下植入羟基磷灰石涂层的纤维素海绵诱导肉芽组织形成。术后1 - 3天观察到大量炎症反应，伴有强烈的异物反应和纤维血管组织浸润增加，而未涂层对照植入物中的组织生长则慢得多，且主要发生在其表面。术后14天后，磷灰石涂层海绵中的异物反应减弱，植入后1个月至1年，两种纤维素海绵之间未见明显差异。磷灰石涂层植入物吸引巨噬细胞和成纤维细胞，并有利于血管生成。过度的结缔组织形成在组织学上是正常的，以正常比例合成主要的细胞外基质分子，并且似乎没有以任何方式干扰动物。当需要结缔组织快速增殖时，这些结果为进一步研究羟基磷灰石涂层纤维素海绵的临床适用性提供了依据。
