Lee Yoo Chul, Ahn Byung Jun, Jin Jong Sook, Kim Jung Uk, Lee Sang Hwa, Song Do Young, Lee Won Kil, Lee Je Chul
Department of Microbiology, Kyungpook National University School of Medicine, Daegu 700-422, Republic of Korea.
J Microbiol. 2007 Aug;45(4):358-63.
Multi-drug resistant Pseudomonas aeruginosa has been implicated in a variety of serious therapeutic problems in clinical environments. Among the 968 P. aeruginosa isolates obtained from two hospitals in Daegu, Korea, we acquired 17 isolates that were resistant to all available tested antimicrobial agents, with the exception of colistin (colistin-only sensitive). We characterized the antimicrobial susceptibilities, metallo-beta-lactamases, and epidemiological relatedness among the colistin-only sensitive P. aeruginosa isolates. All colistin-only sensitive isolates were positive in the modified Hodge test and imipenem-EDTA synergy test, thereby indicating the production of metallo-beta-lactamases. 11 isolates from the secondary hospital and six isolates from the tertiary teaching hospital harbored blaVIM-2 and blaIMP-1, respectively. The pulsed-field gel electrophoretic analysis of the SpeI-digested DNA from P. aeruginosa isolates indicated that two different clones of colistin-only sensitive P. aeruginosa originated from each hospital, and had spread within the hospital environment. Overall, colistin-only sensitive P. aeruginosa was detected in Korea for the first time, but no pan-drug resistant bacteria were identified. Nationwide surveillance is required in order to monitor the emergence of colistin-only sensitive or pan-drug resistant bacteria.
多重耐药铜绿假单胞菌与临床环境中的各种严重治疗问题有关。在从韩国大邱的两家医院获得的968株铜绿假单胞菌分离株中，我们获得了17株对所有可用测试抗菌药物耐药的分离株，但多粘菌素除外（仅对多粘菌素敏感）。我们对仅对多粘菌素敏感的铜绿假单胞菌分离株的抗菌药敏性、金属β-内酰胺酶和流行病学相关性进行了表征。所有仅对多粘菌素敏感的分离株在改良Hodge试验和亚胺培南-EDTA协同试验中均为阳性，从而表明产生了金属β-内酰胺酶。来自二级医院的11株分离株和来自三级教学医院的6株分离株分别携带blaVIM-2和blaIMP-1。对铜绿假单胞菌分离株的SpeI酶切DNA进行脉冲场凝胶电泳分析表明，仅对多粘菌素敏感的铜绿假单胞菌的两个不同克隆分别源自每家医院，并已在医院环境中传播。总体而言，韩国首次检测到仅对多粘菌素敏感的铜绿假单胞菌，但未鉴定出泛耐药菌。需要进行全国范围的监测，以监测仅对多粘菌素敏感或泛耐药菌的出现。
