Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon, South Korea.
Microb Drug Resist. 2011 Jun;17(2):299-304. doi: 10.1089/mdr.2010.0145. Epub 2011 Mar 7.
The in vitro activity of colistin was evaluated against 215 nonduplicated Pseudomonas aeruginosa isolates, including 53 multidrug-resistant isolates, which were collected between 2006 and 2007 from nine tertiary care hospitals in Korea. Colistin-nonsusceptible P. aeruginosa (CNPA) isolates were genotyped using multilocus sequence typing. Sixteen (7.4%) CNPA isolates (minimum inhibitory concentration [MIC], >2 mg/l) were identified, including three resistant isolates. All but one of the MDR P. aeruginosa isolates was susceptible to colistin. Multilocus sequence typing analysis identified 12 sequence types (STs) among 16 CNPA isolates, indicating that colistin nonsusceptibility might arise independently. However, ST244 and ST292, which may be international clones, were found in multiple CNPA isolates. Our data indicate an increase of P. aeruginosa isolates with reduced colistin susceptibility, suggesting the need for continuous surveillance of P. aeruginosa.
对 215 株非重复的铜绿假单胞菌分离株进行了粘菌素的体外活性评估，其中包括 2006 年至 2007 年间从韩国 9 家三级保健医院收集的 53 株多药耐药分离株。使用多位点序列分型对粘菌素不敏感的铜绿假单胞菌（CNPA）分离株进行了基因分型。鉴定出 16 株（7.4%）CNPA 分离株（最小抑菌浓度 [MIC]，>2 mg/l），包括 3 株耐药分离株。除 1 株外，所有多药耐药铜绿假单胞菌分离株均对粘菌素敏感。16 株 CNPA 分离株中的多位点序列分型分析鉴定出 12 种序列型（STs），表明粘菌素耐药性可能独立出现。然而，在多个 CNPA 分离株中发现了 ST244 和 ST292，这可能是国际克隆。我们的数据表明，具有较低粘菌素敏感性的铜绿假单胞菌分离株有所增加，这表明需要对铜绿假单胞菌进行持续监测。
