Costa Júlio César Rocha, Dias Roberto Júnio Pedroso, Morenz Mirton José Frota
Universidade Federal de Juiz de Fora, Campus Universitário, Martelos, CEP 36036-900, Juiz de Fora, Minas Gerais, Brazil.
Parasitol Res. 2007 Dec;102(1):139-44. doi: 10.1007/s00436-007-0747-9. Epub 2007 Sep 12.
Our objective was to determine the mean production of entomopathogenic nematodes (EPNs) infective juveniles (IJs) from Alphitobius diaperinus and Galleria mellonella larvae and the possible morphometric changes of emergent IJs. Heterorhabditis riobravus and Steinernema carpocapsae nematodes were placed on 20 larvae of each host individually located in Petri dishes, which were maintained in an environmental control chamber. After death, each larva was individually transferred to White traps where they remained for a maximum of 20 days in environmental control chambers. With IJ multiplication, the water from each trap was separately collected, emergent IJs were counted, and mean production was calculated for each host species. Relative populations of each nematode species emerging from each host were randomly selected and miscegenated. Then, 50 IJs from each host/species were randomly selected for morphometric studies. Significant difference was seen between the two EPN species for mean IJ production values from G. mellonella larvae (P = 0.0048) but not from A. diaperinus larvae (P = 0.8883). Significant differences were also seen in total body length and width between the emergent H. riobravus and S. carpocapsae IJs (P = 0.0002).
我们的目标是确定从暗黑鳃金龟和大蜡螟幼虫中产生的昆虫病原线虫（EPNs）感染性幼虫（IJs）的平均产量，以及新出现的IJs可能的形态测量变化。将里奥布氏异小杆线虫和小卷蛾斯氏线虫分别置于培养皿中单独放置的20只每种宿主的幼虫上，这些培养皿放置在环境控制室中。幼虫死亡后，将每只幼虫单独转移到怀特诱捕器中，在环境控制室中最多放置20天。随着IJs的繁殖，分别收集每个诱捕器中的水，对新出现的IJs进行计数，并计算每种宿主物种的平均产量。从每种宿主中出现的每种线虫物种的相对种群被随机选择并混合。然后，从每个宿主/物种中随机选择50只IJs进行形态测量研究。对于从大蜡螟幼虫中产生的IJs平均产量值，两种EPN物种之间存在显著差异（P = 0.0048），但从暗黑鳃金龟幼虫中产生的IJs平均产量值无显著差异（P = 0.8883）。新出现的里奥布氏异小杆线虫和小卷蛾斯氏线虫IJs在总体长和宽度上也存在显著差异（P = 0.0002）。
