Programa de Pós-graduação em Ciências Veterinárias, Universidade Federal Rural do Rio de Janeiro, Rodovia BR 465, 23890-000, Seropédica, RJ, Brazil,
Parasitol Res. 2013 Oct;112(10):3645-51. doi: 10.1007/s00436-013-3552-7. Epub 2013 Aug 15.
The aim of the study was to assess the effect of the association of entomopathogenic nematodes and fungi on Rhipicephalus microplus. The nematodes used were Heterorhabditis bacteriophora HP88 and Heterorhabditis indica LPP1 and the fungi were Metarhizium anisopliae IBCB 116 and Beauveria bassiana ESALQ 986. In the groups treated with the fungi, the females were immersed for 3 min in a conidial suspension, while in the groups treated with the nematodes, the ticks were exposed to infective juveniles. To evaluate the interaction between entomopathogens, the females were first immersed in a conidial suspension and then exposed to the nematodes. The egg mass weight and hatching percentage values of the groups treated with M. anisopliae IBCB 116 and B. bassiana ESALQ 986 in the two experiments were statistically similar (p > 0.05) to the values of the control group. In the groups treated only with nematodes, there was a significant reduction (p < 0.05) in the egg mass weight, a fact also observed for the hatching percentage of the group treated with H. indica LPP1. In all the groups treated with nematodes in association with fungi, there was a significant reduction (p < 0.05) in the egg mass weight and hatching percentage. The percentage of control of the groups treated with fungi alone varied from 31 to 55%. In the groups treated with nematodes associated or not with fungi, the control percentage was always greater than 90% and reached 100% in the group treated with H. bacteriophora HP88 associated with the fungus M. anisopliae IBCB 116.
本研究旨在评估昆虫病原线虫和真菌对微小牛蜱的联合作用。使用的线虫为嗜菌异小杆线虫 HP88 和印度异小杆线虫 LPP1，真菌为绿僵菌 IBCB 116 和球孢白僵菌 ESALQ 986。在真菌处理组中，雌性蜱虫浸泡在分生孢子悬浮液中 3 分钟，而在线虫处理组中，蜱虫暴露于感染性幼虫中。为了评估昆虫病原物之间的相互作用，先将雌性蜱虫浸泡在分生孢子悬浮液中，然后再暴露于线虫中。在两个实验中，用绿僵菌 IBCB 116 和球孢白僵菌 ESALQ 986 处理的组的卵块重量和孵化率与对照组的数值统计学上无显著差异（p＞0.05）。在仅用线虫处理的组中，卵块重量显著降低（p＜0.05），用 LPP1 处理的组的孵化率也观察到了同样的结果。在用线虫和真菌联合处理的所有组中，卵块重量和孵化率都显著降低（p＜0.05）。单独用真菌处理的组的控制率在 31%至 55%之间变化。在用线虫联合或不联合真菌处理的组中，控制率始终大于 90%，在用线虫 HP88 联合真菌绿僵菌 IBCB 116 处理的组中达到 100%。
