Levine Daniel, Kaufman Lydia, Cuenca Violeta G, Badawy Shawky Z A
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA.
J Reprod Med. 2007 Jul;52(7):581-4.
To examine the effect of the GnRH agonist leuprolide on the growth of cultured endometrioma cells.
Experimental study in an academic setting on endometrioma cell lines cultured from 15 women undergoing laparoscopy or laparotomy for excision of endometrioma.
Mean cell counts resulting from treatment with lower concentrations were not significantly different from those of the controls. Increasing concentrations of leuprolide resulted in inhibition of cell growth. The inhibitory effect of leuprolide was statistically significant when the 1,000 ng/mL concentration was compared with the control concentration.
Increased concentrations of leuprolide has suppressive effects on the growth of cultured endometrioma cells. This suggests a direct effect of GnRH agonists acting via GnRH agonist receptors. Long-acting gonadotropin-releasing hormone (GnRH) agonists cause pituitary receptor down-regulation and ovarian suppression, a function that has made this class of agents useful for the treatment of endometriosis. Recent work has also revealed that this class of agents may also have a direct suppressive effect on peripheral target tissue, mediated by GnRH and GnRH agonist receptors. Preliminary work has suggested that there are GnRH receptors in endometriotic cells and that the growth of these cells is inhibited by GnRH agonists. This activity, however, has not been extensively studied in the growth of endometrioma cells. The present study evaluated the effect of the GnRH agonist leuprolide on 15 endometrioma cell lines.
研究促性腺激素释放激素（GnRH）激动剂亮丙瑞林对培养的子宫内膜异位囊肿细胞生长的影响。
在学术环境中对15例因子宫内膜异位囊肿接受腹腔镜或剖腹手术切除的女性所培养的子宫内膜异位囊肿细胞系进行的实验研究。
较低浓度治疗后的平均细胞计数与对照组无显著差异。亮丙瑞林浓度增加导致细胞生长受到抑制。当将1000 ng/mL浓度与对照浓度比较时，亮丙瑞林的抑制作用具有统计学意义。
亮丙瑞林浓度增加对培养的子宫内膜异位囊肿细胞生长具有抑制作用。这提示GnRH激动剂通过GnRH激动剂受体发挥直接作用。长效促性腺激素释放激素（GnRH）激动剂可导致垂体受体下调和卵巢抑制，这一功能使这类药物可用于治疗子宫内膜异位症。近期研究还表明，这类药物可能也对外周靶组织具有直接抑制作用，由GnRH和GnRH激动剂受体介导。初步研究提示，子宫内膜异位细胞中存在GnRH受体，且GnRH激动剂可抑制这些细胞的生长。然而，这一作用在子宫内膜异位囊肿细胞生长方面尚未得到广泛研究。本研究评估了GnRH激动剂亮丙瑞林对15个子宫内膜异位囊肿细胞系的影响。
