Wong Kenneth H H, Simon James A
Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Utah Medical Center, Salt Lake City, Utah, USA.
Am J Obstet Gynecol. 2004 Jan;190(1):44-9. doi: 10.1016/j.ajog.2003.08.032.
The purpose of this study was to assess the in vitro effect of gonadotropin-releasing hormone agonist on natural killer cell activity in women with and without endometriosis and to ascertain whether gonadotropin-releasing hormone agonist effects on natural killer cell activity are direct or mediated solely through the hypoestrogenic state that they produce in vivo.
With use of a chromium 51 release microcytotoxicity assay with K562 target cells, natural killer cell activity was measured after the incubation of mononuclear cells with leuprolide acetate that was obtained from 16 patients with endometriosis and 11 control subjects.
The cytotoxicity of natural killer cells that were obtained from patients with endometriosis was reduced significantly (P<.001) with leuprolide. Natural killer cell cytotoxicity from control patients was also significantly decreased (P=.005) with gonadotropin-releasing hormone agonist. Natural killer cell cytotoxicity was significantly lower in patients with endometriosis than in control patients (P=.029).
These findings suggest a direct immunomodulatory role of gonadotropin-releasing hormone agonist on natural killer cell activity and confirm previous findings that patients with endometriosis have reduced natural killer cell cytotoxicity.
本研究旨在评估促性腺激素释放激素激动剂对患有和未患有子宫内膜异位症女性自然杀伤细胞活性的体外作用，并确定促性腺激素释放激素激动剂对自然杀伤细胞活性的影响是直接的还是仅通过其在体内产生的低雌激素状态介导的。
使用铬51释放微量细胞毒性试验，以K562靶细胞为对象，将从16例子宫内膜异位症患者和11例对照受试者获取的单核细胞与醋酸亮丙瑞林孵育后，测量自然杀伤细胞活性。
醋酸亮丙瑞林使子宫内膜异位症患者的自然杀伤细胞细胞毒性显著降低（P<0.001）。促性腺激素释放激素激动剂也使对照患者的自然杀伤细胞细胞毒性显著降低（P=0.005）。子宫内膜异位症患者的自然杀伤细胞细胞毒性显著低于对照患者（P=0.029）。
这些发现提示促性腺激素释放激素激动剂对自然杀伤细胞活性具有直接的免疫调节作用，并证实了先前的研究结果，即子宫内膜异位症患者的自然杀伤细胞细胞毒性降低。
