用于非病毒基因递送的可生物降解微粒上聚酰胺胺树枝状大分子的共轭。

Conjugation of polyamidoamine dendrimers on biodegradable microparticles for nonviral gene delivery.

作者信息

Zhang Xue-Qing, Intra Janjira, Salem Aliasger K

机构信息

Division of Pharmaceutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA.

出版信息

Bioconjug Chem. 2007 Nov-Dec;18(6):2068-76. doi: 10.1021/bc070116l. Epub 2007 Sep 12.

DOI:10.1021/bc070116l

PMID:17848077

Abstract

We report on the preparation and characterization of poly(D, L-lactide-co-glycolide) (PLGA) microparticles with surface-conjugated polyamidoamine (PAMAM) dendrimers of varying generations. The buffering capacity and zeta-potential of the PLGA PAMAM microparticles increased with increasing generation level of the PAMAM dendrimer conjugated. Conjugation of the PAMAM dendrimer to the surface of the PLGA microparticle removed generation-dependent cytotoxicity in HEK293 and COS7 cell lines. PLGA PAMAM pDNA microparticles displayed similar cytotoxicity profiles to unmodified PLGA pDNA microparticles in COS7 cells. A generation three PAMAM dendrimer conjugated to PLGA microparticles significantly increased transfection efficiencies in comparison to unmodified PLGA microparticles.

摘要

我们报道了具有不同代数表面共轭聚酰胺-胺（PAMAM）树枝状大分子的聚（D，L-丙交酯-共-乙交酯）（PLGA）微粒的制备与表征。PLGA-PAMAM微粒的缓冲能力和ζ电位随着共轭PAMAM树枝状大分子代数水平的增加而增加。PAMAM树枝状大分子与PLGA微粒表面的共轭消除了在HEK293和COS7细胞系中与代数相关的细胞毒性。PLGA-PAMAM-pDNA微粒在COS7细胞中显示出与未修饰的PLGA-pDNA微粒相似的细胞毒性特征。与未修饰的PLGA微粒相比，与PLGA微粒共轭的第三代PAMAM树枝状大分子显著提高了转染效率。