Scorei R, Ciubar Raluca, Iancu Cristina, Mitran Valentina, Cimpean Anisoara, Iordachescu Dana
Department of Biochemistry, University of Craiova, 13 A.I. Cuza, Craiova, 200585, Romania.
Biol Trace Elem Res. 2007 Jul;118(1):27-37. doi: 10.1007/s12011-007-0019-7.
Discovery of naturally occurring boron complexes with organic compounds containing hydroxyl groups, sugars, and polysaccharides, adenosine-5-phosphate, pyridoxine, riboflavin, dehydroascorbic acid, and pyridine nucleotides led to the reassessment of the biochemical role of boron. Boron's anti-inflammatory actions were claimed but not yet demonstrated. This study investigated the effects of calcium fructoborate (CF) on the human polymorphonuclear neutrophils (PMN) that play a central role in the inflammatory response. Our results demonstrated that CF exposure induced a dose-dependent decrease in cell viability. Treatment of PMN cells, for 24 h, with 22,500 microM CF led to a decrease in cell viability by 61.1%, an inhibition of respiratory burst by 92.9% in the case of fMLP-stimulated cells, a diminution of intracellular level of superoxide anion with 59.3%, and a stimulation of superoxide dismutase activity by 72% in unstimulated PMN cells. Altogether, these results suggest the antioxidant and anti-inflammatory properties of CF.
天然存在的硼与含羟基有机化合物、糖类、多糖、5'-磷酸腺苷、吡哆醇、核黄素、脱氢抗坏血酸和吡啶核苷酸形成的复合物的发现，促使人们重新评估硼的生化作用。硼具有抗炎作用的说法虽有，但尚未得到证实。本研究调查了果糖硼酸钙（CF）对在炎症反应中起核心作用的人多形核中性粒细胞（PMN）的影响。我们的结果表明，暴露于CF会导致细胞活力呈剂量依赖性下降。用22500微摩尔/升CF处理PMN细胞24小时，导致细胞活力下降61.1%，在fMLP刺激的细胞中呼吸爆发抑制率达92.9%，细胞内超氧阴离子水平降低59.3%，在未刺激的PMN细胞中超氧化物歧化酶活性提高72%。总之，这些结果表明CF具有抗氧化和抗炎特性。
