Steiniger Birte, Stachniss Vitus, Schwarzbach Hans, Barth Peter J
Institute of Anatomy and Cell Biology, University of Marburg, Robert-Koch-Str. 8, 35032, Marburg, Germany.
Histochem Cell Biol. 2007 Nov;128(5):391-8. doi: 10.1007/s00418-007-0320-8. Epub 2007 Sep 12.
The distribution of capillaries, sinuses and larger vessels was investigated by immunohistology in paraffin sections of 12 adult human spleens using a panel of antibodies. Double staining for CD34 and CD141 (thrombomodulin) revealed that capillary endothelia in the cords of the splenic red pulp and at the surface of follicles were CD34(+)CD141(-), while red pulp sinus endothelia had the phenotype CD34(-)CD141(+). Only in the direct vicinity of splenic follicles did sinus endothelial cells exhibit both antigens. Thus, splenic sinuses do not replace conventional capillaries, but exist in addition to such vessels. The endothelium in arterioles, venules and larger arteries and veins was uniformly CD34(+)CD141(+). Anti-CD34 and anti-CD141 both additionally reacted with different types of splenic stromal cells. Differential staining of capillaries and sinuses may permit a three-dimensional reconstruction of serial sections to unequivocally delineate the "open" and "closed" splenic circulation in humans.
利用一组抗体，通过免疫组织学方法对12例成人脾脏石蜡切片中的毛细血管、血窦和较大血管的分布进行了研究。对CD34和CD141（血栓调节蛋白）进行双重染色显示，脾红髓索和滤泡表面的毛细血管内皮为CD34(+)CD141(-)，而红髓血窦内皮的表型为CD34(-)CD141(+)。仅在脾滤泡的紧邻区域，血窦内皮细胞才同时表达这两种抗原。因此，脾血窦并非取代传统毛细血管，而是与这类血管并存。小动脉、小静脉以及较大动脉和静脉的内皮均一致为CD34(+)CD141(+)。抗CD34和抗CD141还与不同类型的脾基质细胞发生反应。毛细血管和血窦的差异染色可能有助于对连续切片进行三维重建，从而明确描绘人类脾脏的“开放”和“封闭”循环。
