Pinkus G S, Warhol M J, O'Connor E M, Etheridge C L, Fujiwara K
Am J Pathol. 1986 Jun;123(3):440-53.
The anatomic distribution of smooth muscle myosin, a contractile protein, was determined in a variety of lymphoid tissues (spleen, lymph nodes, tonsils) with the use of highly specific rabbit antibodies to human uterine smooth muscle myosin and an indirect immunoperoxidase technique. In the spleen, in addition to the anticipated immunoreactivity in the walls of arteries, veins, splenic capsule, and trabeculas, other staining patterns were observed. Smooth muscle myosin-containing cells which comprised the adventitia of the trabecular arteries appeared continuous with myosin-containing reticular cells of the white pulp. The latter cells assumed a circumferential pattern within the periarteriolar lymphoid sheaths, then blended delicately with the red pulp at the marginal zone. Ultrastructurally, immunogold techniques demonstrated that smooth muscle myosin in these cells was localized to cytoplasmic filaments. Within the red pulp, a different and distinct staining pattern was observed for the splenic sinuses. Short, regular, orderly, and repetitive bands of immunoreactivity, aligned parallel to the long axis of the sinus, extended between contiguous ring fibers. By immunoelectron microscopy these structures corresponded to distinct bundles of filaments in the endothelial lining cells of the splenic sinuses. Factor VIII associated antigen was also identified in the splenic lining cells in cryostat and paraffin sections, and ultrastructurally. Within the red pulp of the spleen, the sheaths of sheathed capillaries also revealed strong immunoreactivity for smooth muscle myosin. Other sites of immunohistochemical localization of smooth muscle myosin included dendritic reticulum cells present in reactive follicles and in nodular non-Hodgkin's lymphomas. Certain vascular structures, specifically sinus lining cells and Schweigger-Seidel capillary sheaths of the spleen and postcapillary venules of lymph nodes and tonsils, coexpressed smooth muscle myosin and Factor VIII associated antigen. The patterns of localization of smooth muscle myosin are correlated with anatomic structures and possible tissue functions.
利用针对人子宫平滑肌肌球蛋白的高度特异性兔抗体和间接免疫过氧化物酶技术，确定了收缩蛋白平滑肌肌球蛋白在多种淋巴组织（脾脏、淋巴结、扁桃体）中的解剖分布。在脾脏中，除了在动脉壁、静脉壁、脾包膜和小梁中预期的免疫反应性外，还观察到其他染色模式。构成小梁动脉外膜的含平滑肌肌球蛋白的细胞似乎与白髓中含肌球蛋白的网状细胞连续。后者的细胞在动脉周围淋巴鞘内呈圆周模式，然后在边缘区与红髓巧妙融合。超微结构上，免疫金技术表明这些细胞中的平滑肌肌球蛋白定位于细胞质细丝。在红髓内，脾窦呈现出不同且独特的染色模式。短的、规则的、有序的和重复的免疫反应带平行于窦的长轴排列，在相邻的环纤维之间延伸。通过免疫电子显微镜观察，这些结构对应于脾窦内皮衬里细胞中不同的细丝束。在低温切片和石蜡切片以及超微结构中，还在脾衬里细胞中鉴定出了因子VIII相关抗原。在脾脏的红髓内，被膜毛细血管的鞘对平滑肌肌球蛋白也显示出强烈的免疫反应性。平滑肌肌球蛋白免疫组织化学定位的其他部位包括反应性滤泡和结节性非霍奇金淋巴瘤中存在的树突状网状细胞。某些血管结构，特别是脾脏的窦衬里细胞和施韦格 - 赛德尔毛细血管鞘以及淋巴结和扁桃体的毛细血管后微静脉，共表达平滑肌肌球蛋白和因子VIII相关抗原。平滑肌肌球蛋白的定位模式与解剖结构和可能的组织功能相关。
