Arias Lida, Bauzá Jorge, Tobella Joana, Vila Joaquim, Grifoll Magdalena
Department of Microbiology, University of Barcelona, Diagonal 645, Barcelona 08028, Spain.
Biodegradation. 2008 Jun;19(3):425-34. doi: 10.1007/s10532-007-9148-0. Epub 2007 Sep 12.
During bioremediation of polycyclic aromatic hydrocarbon (PAH)-polluted soils accumulation of polar metabolites resulting from the biological activity may occur. Since these polar metabolites are potentially more toxic than the parental products, a better understanding of the processes involved in the production and fate of these oxidation products in soil is needed. In the present work we describe the design and set-up of a static soil microcosm system and an analytical methodology for detection of PAHs and their oxidation products in soils. When applied to a soil contaminated with phenanthrene, as a model PAH, and 1-hydroxy-2-naphthoic acid, diphenic acid, and phthalic acid as putative metabolites, the extraction and fractionation procedures resulted in recoveries of 93%, 89%, 100%, and 89%, respectively. The application of the standardized system to study the biodegradation of phenanthrene in an agricultural soil with and without inoculation of the high molecular weight PAH-degrading strain Mycobacterium sp. AP1, demonstrates its suitability for determining the environmental fate of PAHs in polluted soils and for evaluating the effect of bioremediative treatments. In inoculated microcosms 35% of the added phenanthrene was depleted, 19% being recovered as CO(2) and 3% as diphenic acid. The latter, together with other two unidentified metabolites, accumulated in soil.
在多环芳烃（PAH）污染土壤的生物修复过程中，可能会出现因生物活性产生的极性代谢物的积累。由于这些极性代谢物可能比母体产物毒性更大，因此需要更好地了解土壤中这些氧化产物的产生和归宿所涉及的过程。在本研究中，我们描述了一个静态土壤微观系统的设计和设置，以及一种用于检测土壤中多环芳烃及其氧化产物的分析方法。当该方法应用于受菲污染的土壤（菲作为多环芳烃模型）以及1 - 羟基 - 2 - 萘甲酸、联苯二甲酸和邻苯二甲酸作为假定代谢物时，提取和分馏程序的回收率分别为93%、89%、100%和89%。将该标准化系统应用于研究农业土壤中菲的生物降解，该土壤接种和未接种高分子量多环芳烃降解菌株分枝杆菌属AP1，结果表明该系统适用于确定污染土壤中多环芳烃的环境归宿以及评估生物修复处理的效果。在接种的微观系统中，添加的菲有35%被消耗，19%以二氧化碳形式回收，3%以联苯二甲酸形式回收。后者与其他两种未鉴定的代谢物一起在土壤中积累。
