Beaudry Francis, Vachon Pascal
Department of Veterinary Biomedicine, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, St-Hyacinthe, Quebec, Canada J2S 2M2.
Biomed Chromatogr. 2008 Jan;22(1):1-4. doi: 10.1002/bmc.906.
Glucosamine is an amino sugar involved in the biosynthesis of glycosylated proteins and lipids. Recently, with increased public interest in natural products medicine, glucosamine has been widely used to treat osteoarthritis, even though demonstrations of its actual efficacy remain relatively unknown. Information related to the pharmcokinetics of glucosamine is sparse. A recent analytical method published used 13C-glucosamine as an internal standard to analyse study samples. The method lacked accuracy owing to an important natural isotopic contribution of glucosamine to 13C-glucosamine ion abundance. This manuscript describes a simple method to quantify glucosamine in horse plasma. Glucosamine was extracted by protein precipitation with acetonotrile containing 0.1% formic acid. The chromatography was performed on a Agilent Hypersil-ODS 100x2.1 mm column with a mobile phase composed of acetonitrile and 0.5% formic acid in water (45:55) at a flow rate of 0.3 mL/min. A linear (1/x) relationship was used to perform the calibration over an analytical range of 10-1000 ng/mL. The inter-batch precision and accuracy ranged from 5.3 to 11.3% and from 87.8 to 107.2% in horse plasma, respectively. The mean endogenous level of glucosamine in horse plasma was 14.4 ng/mL (n=6). This LC-ESI/MS/MS method for the determination of glucosamine in horse plasma provided results within generally accepted criteria used for bioanalytical assay.
氨基葡萄糖是一种参与糖基化蛋白质和脂质生物合成的氨基糖。最近，随着公众对天然产物药物兴趣的增加，氨基葡萄糖已被广泛用于治疗骨关节炎，尽管其实际疗效的证据仍然相对较少。有关氨基葡萄糖药代动力学的信息很少。最近发表的一种分析方法使用13C-氨基葡萄糖作为内标来分析研究样品。由于氨基葡萄糖对13C-氨基葡萄糖离子丰度有重要的天然同位素贡献，该方法缺乏准确性。本文描述了一种定量马血浆中氨基葡萄糖的简单方法。通过用含0.1%甲酸的乙腈进行蛋白质沉淀来提取氨基葡萄糖。色谱分析在安捷伦Hypersil-ODS 100x2.1 mm柱上进行，流动相由乙腈和0.5%甲酸水溶液（45:55）组成，流速为0.3 mL/min。在10-1000 ng/mL的分析范围内，采用线性（1/x）关系进行校准。马血浆中的批间精密度和准确度分别为5.3%至11.3%和87.8%至107.2%。马血浆中氨基葡萄糖的平均内源性水平为14.4 ng/mL（n = 6）。这种用于测定马血浆中氨基葡萄糖的LC-ESI/MS/MS方法提供的结果在生物分析测定的普遍接受标准范围内。
