Niehuis O, Judson A K, Werren J H, Hunter W B, Dang P M, Dowd S E, Grillenberger B, Beukeboom L W, Gadau J
School of Life Sciences, Arizona State University, P.O. Box 874501, Tempe, AZ 85287-4501, USA.
J Econ Entomol. 2007 Aug;100(4):1033-6. doi: 10.1603/0022-0493(2007)100[1033:sspass]2.0.co;2.
Wasps of the genus Nasonia are important biological control agents of house flies and related filth flies, which are major vectors of human pathogens. Species of Nasonia (Hymenoptera: Pteromalidae) are not easily differentiated from one another by morphological characters, and molecular markers for their reliable identification have been missing so far. Here, we report eight single-nucleotide polymorphism and three sequence-tagged site markers derived from expressed sequenced tag libraries for the two closely related and regionally sympatric species N. giraulti and N. vitripennis. We studied variation of these markers in natural populations of the two species, and we mapped them in the Nasonia genome. The markers are species-diagnostic and evenly spread over all five chromosomes. They are ideal for rapid species identification and hybrid recognition, and they can be used to map economically relevant quantitative trait loci in the Nasonia genome.
丽蝇蛹集金小蜂属的黄蜂是家蝇及相关腐食性蝇类的重要生物防治媒介，而这些蝇类是人类病原体的主要传播者。丽蝇蛹集金小蜂属（膜翅目：金小蜂科）的物种不易通过形态特征相互区分，并且迄今为止一直缺少用于可靠鉴定它们的分子标记。在此，我们报告了从表达序列标签文库中获得的八个单核苷酸多态性标记和三个序列标签位点标记，这些标记来自两个亲缘关系密切且在区域上同域分布的物种——吉氏丽蝇蛹集金小蜂和玻璃丽蝇蛹集金小蜂。我们研究了这两个物种自然种群中这些标记的变异情况，并将它们定位到丽蝇蛹集金小蜂的基因组中。这些标记具有物种诊断性，并且均匀地分布在所有五条染色体上。它们非常适合快速进行物种鉴定和杂种识别，并且可用于在丽蝇蛹集金小蜂基因组中定位与经济相关的数量性状位点。
