Salvador Ana M, Alonso-Damián Ana, Choubert Georges, Milicua José Carlos G
Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Spain.
J Agric Food Chem. 2007 Oct 31;55(22):9202-7. doi: 10.1021/jf070145q. Epub 2007 Sep 12.
This study was designed to assess the effect of dietary soybean phospholipids on canthaxanthin transport by serum lipoproteins and canthaxanthin muscle deposition in trout. Three groups of 12 immature trout in triplicate with a mean body weight of 130 g were fed with three experimental diets containing (1) canthaxanthin plus lecithin plus fish oil, (2) canthaxanthin plus lecithin, and (3) canthaxanthin alone, for 12 days. The two major lipoprotein classes in rainbow trout are high-density lipoproteins, which transport principally carotenoids present in the serum, and low-density lipoproteins, which are responsible for the transport of cholesterol, both independently of the administered diet. In addition, very low density lipoproteins are responsible for triglyceride transport in serum. Nevertheless, the amount of canthaxanthin in the serum increased when carotenoid was associated with phospholipids plus fish oil. When canthaxanthin is transported by lecithin plus fish oil, the amount of phospholipids, cantaxanthin, and cholesterol deposited in muscle increased but not significantly. The highest apparent canthaxanthin digestibility coefficient was obtained when canthaxanthin was carried by lecithin plus fish oil. The administration of canthaxanthin carried by phospholipids improved its accumulation in the muscle of rainbow trout. This accumulation could be enhanced if the time of administration of canthaxanthin is increased.
本研究旨在评估日粮大豆磷脂对虹鳟血清脂蛋白转运角黄素以及角黄素在虹鳟肌肉中沉积的影响。将三组12尾平均体重130克的未成熟虹鳟，每组设三个重复，分别投喂三种实验日粮，日粮分别为：(1) 角黄素加卵磷脂加鱼油；(2) 角黄素加卵磷脂；(3) 仅角黄素，投喂12天。虹鳟体内的两种主要脂蛋白类别为高密度脂蛋白，主要负责转运血清中存在的类胡萝卜素，以及低密度脂蛋白，主要负责胆固醇的转运，二者均不受所投喂日粮的影响。此外，极低密度脂蛋白负责血清中甘油三酯的转运。然而，当类胡萝卜素与磷脂加鱼油结合时，血清中的角黄素含量会增加。当角黄素由卵磷脂加鱼油转运时，肌肉中沉积的磷脂、角黄素和胆固醇的量有所增加，但不显著。当角黄素由卵磷脂加鱼油携带时，获得了最高的表观角黄素消化系数。磷脂携带的角黄素给药可改善其在虹鳟肌肉中的积累。如果增加角黄素的给药时间，这种积累可能会增强。
