Saddoud O, Chatti K, Salhi-Hannachi A, Mars M, Rhouma A, Marrakchi M, Trifi M
Laboratoire de Génétique moléculaire, Immunologie et Biotechnologie, Faculté des Sciences de Tunis, Campus universitaire, El Manar, Tunis, Tunisia.
Hereditas. 2007 Sep;144(4):149-57. doi: 10.1111/j.2007.0018-0661.01967.x.
The present study portrays the achievement of the genetic polymorphism surveying and the establishment of an ecotypes identification key on the basis of simple sequence repeats data. Seventy-two Tunisian fig ecotypes in situ and ex situ conserved were analyzed using six microsatellite loci. A total of 58 alleles and 124 genotypes were revealed and permitted to evidence high degree of genetic diversity mainly explained at the intra group level. Cluster analysis based on genetic distances proved that a typical continuous genetic diversity characterizes the local germplasm. In addition, the microsatellite multilocus genotyping has permitted to unambiguously distinguish 70 well-defined ecotypes (resolving power of 97.22%). Data are discussed in relation with the reliability of the used markers to check the conformity of the plant material and to rationally manage the conservation of this crop.
本研究描述了基于简单序列重复数据进行遗传多态性调查以及建立生态型鉴定关键指标的成果。使用六个微卫星位点对72个原位和异位保存的突尼斯无花果生态型进行了分析。共揭示了58个等位基因和124种基因型，并证明存在高度的遗传多样性，主要在组内水平得到解释。基于遗传距离的聚类分析证明，典型的连续遗传多样性是当地种质的特征。此外，微卫星多位点基因分型能够明确区分70个定义明确的生态型（分辨力为97.22%）。结合所用标记的可靠性对数据进行了讨论，以检查植物材料的一致性并合理管理该作物的保护工作。
