Yaguchi Yuichiro, Wada Kota, Uchimizu Hirotaka, Tanaka Yasuhiro, Kojima Hiromi, Moriyama Hiroshi
Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan.
Acta Otolaryngol. 2007 Oct;127(10):1038-44. doi: 10.1080/00016480701200285.
Artificial middle ear mucosa (AMEM), a sheet of mucosal cells grown on collagen gel populated with fibroblasts, is useful as graft material that is able to promote mucosal regeneration after middle ear surgery.
Regeneration of the middle ear mucosa and pneumatization of the mastoid cavity is critical for good prognosis. We examined whether implantation of AMEM into damaged middle ear cavity would promote mucosal regeneration.
AMEM was prepared as described previously using epithelial cells and fibroblasts isolated from the rabbit middle ear. We implanted AMEM into rabbit middle ear from which mucosa had been surgically removed and evaluated its histological and functional recovery 8 weeks later. Three other groups were used for comparison: a normal control group, a mucosa-eliminated group, and a collagen-implanted group.
AMEM grew to be morphologically similar to the native middle ear mucosa. Electron microscope studies showed that implanted AMEM has basal lamina and cilia. AMEM implantation suppressed bone hyperplasia and granulation, leading to better mucosal regeneration. Mucosal gas exchange was also significantly improved after implantation.
人工中耳黏膜（AMEM）是一种在富含成纤维细胞的胶原凝胶上生长的黏膜细胞片，作为一种能够促进中耳手术后黏膜再生的移植材料很有用。
中耳黏膜再生和乳突腔气化对良好预后至关重要。我们研究了将AMEM植入受损中耳腔是否会促进黏膜再生。
按照先前所述方法，使用从兔中耳分离的上皮细胞和成纤维细胞制备AMEM。我们将AMEM植入已通过手术去除黏膜的兔中耳，并在8周后评估其组织学和功能恢复情况。另外三组用于比较：正常对照组、黏膜清除组和胶原植入组。
AMEM生长后在形态上与天然中耳黏膜相似。电子显微镜研究表明，植入的AMEM有基膜和纤毛。AMEM植入抑制了骨质增生和肉芽形成，从而实现了更好的黏膜再生。植入后黏膜气体交换也显著改善。
