采用高效液相色谱法测定的精氨酸、不对称二甲基精氨酸（ADMA）和对称二甲基精氨酸（SDMA）的健康相关参考值。

Health-associated reference values for arginine, asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) measured with high-performance liquid chromatography.

作者信息

Hov G Garmo, Sagen E, Bigonah A, Asberg A

机构信息

Department of Medical Biochemistry, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.

出版信息

Scand J Clin Lab Invest. 2007;67(8):868-76. doi: 10.1080/00365510701429836.

DOI:10.1080/00365510701429836

PMID:17852822

Abstract

OBJECTIVE

Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of nitric oxide production. It has been implicated in various pathological processes, especially processes involving the cardiovascular system. Plasma concentration of ADMA (p-ADMA) has been measured in several studies with different techniques, and a wide range of normal values has been published. With use of high performance liquid chromatography (HPLC) with fluorescence detection, we have established health-associated reference values for p-ADMA, p-arginine, p-SDMA and the p-arginine/p-ADMA and p-arginine/p-SDMA ratios.

MATERIAL AND METHODS

Blood samples were taken from 238 blood donors (112 F and 126 M). HPLC was used to analyse arginine, ADMA and SDMA. Reference values are presented as 2.5 and 97.5 percentiles estimated by the bootstrap method.

RESULTS

The reference interval for p-ADMA is 0.40-0.77 micromol/L for the whole population, 0.41-0.79 micromol/L for men, 0.38-0.73 micromol/L for women 45 years or less, and 0.41-0.84 micromol/L for women >45 years.

CONCLUSIONS

Age affects p-ADMA in healthy women, but not in men.

摘要

目的

不对称二甲基精氨酸（ADMA）是一氧化氮生成的内源性抑制剂。它与多种病理过程有关，尤其是涉及心血管系统的过程。在多项研究中已采用不同技术测量了血浆ADMA浓度（p-ADMA），并且已公布了广泛的正常参考值范围。通过使用带荧光检测的高效液相色谱法（HPLC），我们建立了与健康相关的p-ADMA、p-精氨酸、p-SDMA以及p-精氨酸/p-ADMA和p-精氨酸/p-SDMA比值的参考值。