克里特岛一家综合医院的分枝杆菌物种多样性：希腊首次检测到缓黄分枝杆菌。

Mycobacterial species diversity at a general hospital on the island of Crete: first detection of Mycobacterium lentiflavum in Greece.

作者信息

Neonakis Ioannis K, Gitti Zoe, Kourbeti Irene S, Michelaki Helen, Baritaki Maria, Alevraki Georgia, Papadomanolaki Evangelia, Tsafaraki Ekaterini, Tsouri Anna, Baritaki Stavroula, Krambovitis Elias, Spandidos Demetrios A

机构信息

Mycobacteriology Laboratory, Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, 712 01 Heraklion, Crete, Greece.

出版信息

Scand J Infect Dis. 2007;39(10):875-9. doi: 10.1080/00365540701402962.

DOI:10.1080/00365540701402962

PMID:17852893

Abstract

The objective of the present study was to investigate the diversity of mycobacterial isolates in a general hospital in Crete, Greece. 48 positive Lowenstein-Jensen cultures over a 3-y period were analysed by means of AccuProbe and GenoType assays. Non-tuberculous mycobacteria (NTM) comprised the majority of the isolates (56.3%, 27/48) vs 33.3% (16/48) of M. tuberculosis; 10.4% of the isolates could not be classified. Among NTM, M. lentiflavum was the predominant species isolated (9/27) followed by M. kansasii, M. gordonae and M. peregrinum, whereas no M. avium complex isolates were detected. This is the first detection of M. lentiflavum in Greece. The susceptibilities of the M. lentiflavum isolates to an extended panel of antibiotics were determined by the proportions method and the medical files of the 9 patients were reviewed. Three isolates were from urine, which is an unusual site. All strains exhibited multidrug resistance. The patients were adults with immunosuppression or predisposing conditions for NTM infection. Diagnosis of true infection was either not pursued or the patients died shortly after isolation.

摘要

本研究的目的是调查希腊克里特岛一家综合医院分枝杆菌分离株的多样性。通过AccuProbe和GenoType检测法对3年期间48份阳性的罗氏培养基培养物进行了分析。非结核分枝杆菌（NTM）占分离株的大多数（56.3%，27/48），而结核分枝杆菌占33.3%（16/48）；10.4%的分离株无法分类。在NTM中，缓黄分枝杆菌是分离出的主要菌种（9/27），其次是堪萨斯分枝杆菌、戈登分枝杆菌和偶发分枝杆菌，未检测到鸟分枝杆菌复合群分离株。这是希腊首次检测到缓黄分枝杆菌。通过比例法测定了缓黄分枝杆菌分离株对一组扩展抗生素的敏感性，并查阅了9例患者的病历。3份分离株来自尿液，这是一个不寻常的部位。所有菌株均表现出多重耐药性。患者为患有免疫抑制或NTM感染易感疾病的成年人。要么未进行真正感染的诊断，要么患者在分离后不久死亡。