Rocha V M, Calil C M, Ferreira R, Moura M J C S, Marcondes F K
Faculty of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
Stress. 2007 Nov;10(4):326-31. doi: 10.1080/10253890701281344.
The aim of this study was to evaluate the influence of nandrolone decanoate on anxiety levels in rats. Male Wistar rats were treated with nandrolone decanoate (5mg/kg, two times per week, i.m.) or vehicle (propylene glycol--0.2 ml/kg, two times per week, IM) for 6 weeks. Control rats were subject only to procedures related to their routine husbandry. By the end of 6 weeks, all groups (24-29 rats/group) were submitted to the elevated plus maze test in order to evaluate their anxiety level. Some of these animals (12-14/group) were treated with diazepam (1 mg/kg i.p.) 30 min before the elevated plus maze test. Nandrolone decanoate significantly decreased the percentage of time spent in the open arms (1.46+/-0.49%) compared with control (3.80+/-0.97%) and vehicle (3.96+/-0.85%) groups, with no difference between control and vehicle treatments. The percentage of open arm entries was also reduced in the group treated with nandrolone decanoate in comparison with the vehicle and control. No changes in the number of closed arm entries were detected. Diazepam abolished the effects of nandrolone decanoate on the percentage of time in, and entries into the open arms. The present study showed that chronic treatment with a high dose of nandrolone decanoate increased the anxiety level in male rats.
本研究旨在评估癸酸诺龙对大鼠焦虑水平的影响。将雄性Wistar大鼠用癸酸诺龙（5mg/kg，每周两次，肌肉注射）或赋形剂（丙二醇——0.2ml/kg，每周两次，肌肉注射）处理6周。对照大鼠仅接受与常规饲养相关的操作。在6周结束时，所有组（每组24 - 29只大鼠）接受高架十字迷宫试验以评估其焦虑水平。其中一些动物（每组12 - 14只）在高架十字迷宫试验前30分钟腹腔注射地西泮（1mg/kg）。与对照组（3.80±0.97%）和赋形剂组（3.96±0.85%）相比，癸酸诺龙显著降低了在开放臂停留的时间百分比（1.46±0.49%），对照组和赋形剂组之间无差异。与赋形剂组和对照组相比，癸酸诺龙处理组的进入开放臂次数百分比也降低。未检测到进入封闭臂次数的变化。地西泮消除了癸酸诺龙对在开放臂停留时间百分比和进入开放臂次数的影响。本研究表明，高剂量癸酸诺龙的慢性处理会增加雄性大鼠的焦虑水平。
