Anatomy Department, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Basic Clin Pharmacol Toxicol. 2010 Apr;106(4):324-30. doi: 10.1111/j.1742-7843.2009.00495.x. Epub 2009 Dec 7.
Anabolic-androgenic steroids are used at high doses by athletes for improving athletic ability, physical appearance and muscle mass. Unfortunately, the abuse of these agents has significantly increased. It has been established that exercise and high doses of anabolic-androgenic steroids may influence the hypothalamic-pituitary-gonadal axis, which can in turn affect testicular apoptosis. However, the effect of the combination of exercise and high dose of anabolic-androgenic steroids on testicular apoptosis is not known. We investigated the combined effects of exercise and high doses of nandrolone decanoate on apoptosis in the spermatogenic cell lineage. Five groups of male Wistar strain albino rats were treated as follows for 8 weeks: solvent of nandrolone decanoate (peanut oil) as a vehicle (Sham); nandrolone decanoate (10 mg/kg/weekly) (nandrolone decanoate); exercise (1 hr/day, 5 days a week) (exercise); nandrolone decanoate (10 mg/kg/weekly) and exercise (1 hr/day, 5 days a week) (nandrolone decanoate exercise); and sedentary control without any injection or exercise (Control). Apoptosis in the male germ line was characterized by TUNEL, caspase-3 assay and transmission electron microscopy. The weights of the testis and accessory sex organs, as well as sperm parameters significantly decreased in the experimental groups relative to the sham and control groups (p < or = 0.05). Germ cell apoptosis and a significant decrease in the number of germ cell layers in nandrolone decanoate exercise-treated testes were observed (p < or = 0.05). Exercise training seems to increase the extent of apoptotic changes caused by supraphysiological dose of nandrolone decanoate in rats, which in turn affects fertility.
合成代谢雄激素类固醇被运动员大剂量用于提高运动能力、身体外貌和肌肉质量。不幸的是，这些药物的滥用显著增加。已经证实，运动和大剂量的合成代谢雄激素类固醇可能会影响下丘脑-垂体-性腺轴，从而影响睾丸细胞凋亡。然而，运动和大剂量合成代谢雄激素类固醇联合对睾丸细胞凋亡的影响尚不清楚。我们研究了运动和大剂量癸酸诺龙联合对生殖细胞谱系细胞凋亡的影响。五组雄性白化 Wistar 大鼠以如下方式处理 8 周：癸酸诺龙的溶剂（花生油）作为载体（假手术）；癸酸诺龙（10mg/kg/周）（癸酸诺龙）；运动（1 小时/天，每周 5 天）（运动）；癸酸诺龙（10mg/kg/周）和运动（1 小时/天，每周 5 天）（癸酸诺龙运动）；以及无任何注射或运动的久坐对照组（对照）。通过 TUNEL、半胱天冬酶-3 测定和透射电镜观察雄性生殖细胞系中的凋亡。实验组的睾丸和附属生殖器官的重量以及精子参数与假手术组和对照组相比显著降低（p < or = 0.05）。在癸酸诺龙运动处理的睾丸中观察到生殖细胞凋亡和生殖细胞层数量显著减少（p < or = 0.05）。运动训练似乎增加了超生理剂量的癸酸诺龙在大鼠中引起的凋亡变化的程度，从而影响生育能力。
