Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia.
Department of Chemistry, Faculty of Science, University of Kragujevac, Radoja Domanovica 12, 34000, Kragujevac, Serbia.
Mol Cell Biochem. 2017 Sep;433(1-2):41-50. doi: 10.1007/s11010-017-3014-0. Epub 2017 Mar 24.
In a last few decades, anabolic-androgenic steroids (AASs) abuse has become serious health concern especially among adolescents. AASs abuse has been reported to be involved in pathogenesis of various mood disorders, including depression. In order to evaluate the effects of chronic (6 weeks) testosterone enanthate (TE) treatment in supraphysiological dose and exercise on depression-like behavior in rats, 32 male rats were divided into four groups: control (C), testosterone enanthate (T, 20 mg/kg/w, s.c.), exercise (E, swimming for 1 h/day), and combined group-testosterone enanthate plus exercise (T + E). TE produced prodepressant effect in tail suspension test (TST) parameters compared to the control and exercise groups, while exercise induced the opposite effect. Simultaneous TE administration along with exercise attenuated the antidepressant effect of exercise reversing the parameters of TST to the control values. Oxidative stress markers in rat hippocampus were significantly altered following applied protocols. TE administration increased index of lipid peroxidation (TBARS) and decreased superoxide dismutase activity (SOD), while exercise induced the opposite effect, with no change in glutathione (GSH) levels. Our results indicate that TE chronic treatment resulted in clear depressive-like behavior, even abolishing beneficial antidepressant effects of exercise in TST that was accompanied with increased oxidative damage in rat hippocampus. The antidepressant effect of exercise correlated with the improvement of redox status in hippocampal tissue. Behavioral parameters obtained in TST significantly correlated with the levels of oxidative stress markers.
在过去的几十年中，合成代谢雄激素类固醇（AAS）滥用已成为一个严重的健康问题，尤其是在青少年中。据报道，AAS 滥用与各种情绪障碍的发病机制有关，包括抑郁症。为了评估慢性（6 周）庚酸睾酮（TE）治疗在超生理剂量和运动对大鼠抑郁样行为的影响，将 32 只雄性大鼠分为 4 组：对照组（C）、庚酸睾酮（T，20mg/kg/w，sc）、运动组（E，每天游泳 1 小时）和联合组-庚酸睾酮加运动（T+E）。与对照组和运动组相比，TE 在悬尾试验（TST）参数中产生促抑郁作用，而运动则产生相反的作用。同时给予 TE 加运动减弱了运动的抗抑郁作用，使 TST 的参数恢复到对照值。大鼠海马中的氧化应激标志物发生了明显变化。TE 给药增加了脂质过氧化（TBARS）指数并降低了超氧化物歧化酶活性（SOD），而运动则产生相反的作用，谷胱甘肽（GSH）水平没有变化。我们的结果表明，TE 慢性治疗导致明显的抑郁样行为，甚至消除了 TST 中运动的有益抗抑郁作用，同时伴有大鼠海马中氧化损伤增加。运动的抗抑郁作用与海马组织氧化还原状态的改善相关。TST 中获得的行为参数与氧化应激标志物的水平显著相关。
