Pérez De Sevilla Müller Luis, Shelley Jennifer, Weiler Reto
Department of Neurobiology, University of Oldenburg, D-26111 Oldenburg, Germany.
J Comp Neurol. 2007 Nov 10;505(2):177-89. doi: 10.1002/cne.21487.
The aim of this study was to characterize and classify the displaced amacrine cells in the mouse retina. Amacrine cells in the ganglion cell layer were injected with fluorescent dyes in flat-mounted retinas. Dye-filled displaced amacrine cells were classified according to dendritic field size, horizontal and vertical stratification patterns, and general morphology. We identified 10 different morphological types of displaced amacrine cell. Six of the cell types identified here are novel cell types that have not been described previously in the mouse retina, to the best of our knowledge. The displaced amacrine cells included four types of medium-field cells, with dendritic field diameters of 200-500 microm, and six types of wide-field cells, with dendritic fields extending over 500 microm. Narrow-field displaced amacrine cells, with dendritic field diameters smaller than 200 microm, were not encountered. The most frequently labeled displaced amacrine cell type was the starburst amacrine cell. At least three cell types identified here have nondisplaced counterparts in the inner nuclear layer as well. Displaced amacrine cells display a rich variety of stratification and branching patterns, which surely reflect the wide range of their functional roles in the processing of visual signals in the inner retina.
本研究的目的是对小鼠视网膜中移位无长突细胞进行特征描述和分类。在平铺的视网膜中，向神经节细胞层中的无长突细胞注射荧光染料。根据树突野大小、水平和垂直分层模式以及总体形态对充满染料的移位无长突细胞进行分类。我们鉴定出10种不同形态类型的移位无长突细胞。据我们所知，这里鉴定出的6种细胞类型是小鼠视网膜中以前未曾描述过的新型细胞类型。移位无长突细胞包括4种中等野细胞，其树突野直径为200 - 500微米，以及6种宽野细胞，其树突野延伸超过500微米。未发现树突野直径小于200微米的窄野移位无长突细胞。最常被标记的移位无长突细胞类型是星爆无长突细胞。这里鉴定出的至少3种细胞类型在内核层也有未移位的对应细胞。移位无长突细胞表现出丰富多样的分层和分支模式，这无疑反映了它们在内视网膜视觉信号处理中广泛的功能作用。
