Bigatti M P, Santovito A
Department of Animal and Human Biology, University of Turin, Italy 10123.
Genetika. 2007 Jun;43(6):827-30.
Glutathione S-transferase is a group of multifunctional enzymes important in the metabolism of xenobiotics. GSTT1 and GSTM1 are polymorphic in human populations. Since a relation between polymorphism and cancer susceptibility has been found, their distribution in human populations is of great interest. In the present study the distribution of GSTT1 and GSTM1 genotypes was studied in a total sample of 252 individuals of three localities of north-west Italy (Postua, Cavaglià and Biella) by PCR test. The frequencies of GSTT1 and GSTM1 "null" genotypes were respectively 7.94% and 34.92%. There are no significant differences between the populations studied in the GSTT1 "null" genotypes. On the other hand, for GSTM1 the frequency of gene deletion in Postua (25.5%) differs significantly (p < 0.01; chi-square test) from that of Biella (46.32%), which approaches the values indicated by most studies for Europeans (about 50%). The analysis of the frequencies of GSTT1 and GSTM1 polymorphisms among different age groups showed a lower frequency of negative genotypes in the older group, although not statistically confirmed.
谷胱甘肽S-转移酶是一组在异源生物代谢中起重要作用的多功能酶。GSTT1和GSTM1在人群中具有多态性。由于已发现多态性与癌症易感性之间的关系，它们在人群中的分布备受关注。在本研究中，通过聚合酶链反应（PCR）检测，对意大利西北部三个地区（波斯图阿、卡瓦利亚和比耶拉）的252名个体的总样本进行了GSTT1和GSTM1基因型分布的研究。GSTT1和GSTM1“无效”基因型的频率分别为7.94%和34.92%。在所研究的人群中，GSTT1“无效”基因型之间没有显著差异。另一方面，对于GSTM1，波斯图阿（25.5%）的基因缺失频率与比耶拉（46.32%）的频率有显著差异（p < 0.01；卡方检验），比耶拉的频率接近大多数欧洲人研究表明的值（约50%）。对不同年龄组中GSTT1和GSTM1多态性频率的分析表明，老年组中阴性基因型的频率较低，尽管未得到统计学证实。
