Mo Xiao-yang, Ma Wenli, Zhang Yali, Zhao Haiquan, Deng Yun, Yuan Wuzhou, Wang Yuequn, Li Yongqin, Zhu Chuanbing, Liu Mingyao, Wu Xiushan
Center for Heart Development, Key Lab of National Education Ministry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, China.
J Med Virol. 2007 Nov;79(11):1783-91. doi: 10.1002/jmv.20942.
Changes in mRNA expression levels of ECV304 cells infected with the wild-type rubella strain were analyzed using a microarray system representing 18,716 human genes. Four hundred eighty-seven genes exhibited differential expression levels; 456 of these genes were up-regulated while 31 genes were down-regulated. We identified 53 biological processes that were significantly relevant to the RV-infection. Among these biological processes, 52 were one-gene processes and one was a process involving five genes: IFNA21 (interferon, alpha 21), interferon stimulated exonuclease gene 20 kDa (ISG20), zinc finger protein 175 (ZNF175), tripartite motif-containing 22 (TRIM22), and MX2 [myxovirus (influenza virus) resistance 2 (mouse)]. Except for ZNF175, gene annotation indicated four of these genes encoded interferon or interferon-induced genes. These results suggest that genes relevant to interferon-regulated pathways may be involved in the pathogenesis of rubella.
使用代表18716个人类基因的微阵列系统分析了感染野生型风疹病毒株的ECV304细胞中mRNA表达水平的变化。487个基因表现出差异表达水平；其中456个基因上调，31个基因下调。我们鉴定出53个与风疹病毒感染显著相关的生物学过程。在这些生物学过程中，52个是单基因过程，1个是涉及5个基因的过程：IFNA21（干扰素，α21）、干扰素刺激的核酸外切酶基因20 kDa（ISG20）、锌指蛋白175（ZNF175）、含三联基序蛋白22（TRIM22）和MX2 [黏液病毒（流感病毒）抗性2（小鼠）]。除ZNF175外，基因注释表明这些基因中的4个编码干扰素或干扰素诱导基因。这些结果表明，与干扰素调节途径相关的基因可能参与风疹的发病机制。
