异黄酮通过雌激素受体调控靶基因转录的作用
[Effect of isoflavones in regulating the transcription of target genes through estrogen receptors].
作者信息
Meng Qing-shu, Zhu Xiao-yan, Tang Xiao-lu, Ma Bei, Ni Xin
机构信息
Department of Physiology, College of Basic Medical Science, Second Military Medical University, Shanghai 200433, China.
出版信息
Zhong Xi Yi Jie He Xue Bao. 2007 Sep;5(5):577-80. doi: 10.3736/jcim20070521.
OBJECTIVE
To observe the transcriptional regulation of the two isoflavones genistein and daidzein on target genes.
METHODS
In this study, we used ERalpha or ERbeta over-expressing Hela cells to observe the transcriptional regulation of genistein and daidzein on ERE reporter gene with calcium-phosphate method, and furthermore observing the effects of phytoestrogen antagonist ICI 182780 on their activation.
RESULTS
Our results showed that both genistein and daidzein could activate ERE receptor gene through ERalpha and ERbeta, and these effects could be blocked by ICI 182780.
CONCLUSION
Both genistein and daidzein can mimic estrogen's effect to activate the transcription of target genes through binding to the ERs.
目的
观察两种异黄酮染料木黄酮和大豆苷元对靶基因的转录调控作用。
方法
在本研究中，我们使用过表达ERα或ERβ的Hela细胞，采用磷酸钙法观察染料木黄酮和大豆苷元对雌激素反应元件（ERE）报告基因的转录调控作用，并进一步观察植物雌激素拮抗剂ICI 182780对它们激活作用的影响。
结果
我们的结果表明，染料木黄酮和大豆苷元均可通过ERα和ERβ激活ERE受体基因，且这些作用可被ICI 182780阻断。
结论
染料木黄酮和大豆苷元均可通过与雌激素受体结合来模拟雌激素的作用，从而激活靶基因的转录。
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