Hayashi Tomomi, Sano Toshio, Kutsuna Natsumaro, Kumagai-Sano Fumi, Hasezawa Seiichiro
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba, 277-8562 Japan.
Plant Cell Physiol. 2007 Oct;48(10):1509-13. doi: 10.1093/pcp/pcm117. Epub 2007 Sep 12.
Anaphase can be categorized into the two subphases of anaphase A and B, but anaphase B has not been clearly described in higher plant cells. In this study, we time-sequentially followed the dynamics of chromosome segregation and spindle elongation in tobacco BY-2 cells using histone-red fluorescent protein (RFP) and green fluorescent protein (GFP)-tubulin, respectively. Construction of kymographs and determination of the positions of chromosomes and spindle edges by image processing revealed that anaphase B contributed to about 40% of the chromosome separation in distance, which is comparable with that in animal cells. These results suggest that higher plant cells potentially possess the process of anaphase B.
后期可分为后期A和后期B两个亚阶段，但高等植物细胞中的后期B尚未得到清晰描述。在本研究中，我们分别使用组蛋白红色荧光蛋白（RFP）和绿色荧光蛋白（GFP）微管蛋白，对烟草BY-2细胞中染色体分离和纺锤体伸长的动态过程进行了时间序列跟踪。通过构建波形图以及利用图像处理确定染色体和纺锤体边缘的位置，结果显示后期B在染色体分离的距离上贡献了约40%，这与动物细胞中的情况相当。这些结果表明高等植物细胞可能具有后期B过程。
