Henderson Gregory C, Fattor Jill A, Horning Michael A, Faghihnia Nastaran, Johnson Matthew L, Mau Tamara L, Luke-Zeitoun Mona, Brooks George A
Exercise Physiology Laboratory, Department of Integrative Biology, University of California, Berkeley, CA 94720-3140, USA.
J Physiol. 2007 Nov 1;584(Pt 3):963-81. doi: 10.1113/jphysiol.2007.137331. Epub 2007 Sep 13.
We sought to determine whether lipolysis, fatty acid (FA) mobilization, and plasma FA oxidation would remain elevated for hours following isoenergetic exercise bouts of different intensities. Ten men and eight women received a primed-continuous infusion of [1,1,2,3,3-(2)H(5)]glycerol and continuous infusion of [1-(13)C]palmitate to measure glycerol and plasma FA kinetics. On Day 1 (D1), participants were studied under one of three different conditions, assigned in random order: (1) before, during and 3 h after 90 min of exercise at 45% V(O2)peak (E45), (2) before, during and 3 h after 60 min of exercise at 65% V(O2)peak (E65), and (3) in a time-matched sedentary control trial (C). For each condition, participants were studied by indirect calorimetry the following morning as well (D2). Rate of appearance (Ra) of glycerol (Ra(GL)) increased above C during exercise in men and women (P < 0.05), was higher in E45 than E65 in men (P < 0.05), and was not different between exercise intensities in women. During 3 h of postexercise recovery, Ra(GL) remained significantly elevated in men (P < 0.05), but not women. FA Ra (Ra(FA)) increased during exercise in men and women and was higher in E45 than E65 (P < 0.05), and remained elevated during 3 h of postexercise recovery in both sexes (P < 0.05), but with a greater relative increase in men than women (P < 0.05). Plasma FA oxidation (Rox) increased during exercise with no difference between intensities, and it remained elevated during 3 h of postexercise recovery in both sexes (P < 0.05). Total lipid oxidation (Lox) was elevated in both sexes (P < 0.05), but more in men during 3 h of postexercise recovery on D1 (P < 0.05) and remained elevated on D2 in men (P < 0.05), but not in women. There were no differences between E45 and E65 for postexercise energy substrate turnover or oxidation in men and women as energy expenditure of exercise (EEE) was matched between bouts. We conclude that the impact of exercise upon lipid metabolism persists into recovery, but that women depend more on lipid during exercise whereas, during recovery, lipid metabolism is accentuated to a greater extent in men.
我们试图确定，在进行不同强度的等能量运动后，脂肪分解、脂肪酸（FA）动员及血浆FA氧化是否会在数小时内持续升高。10名男性和8名女性接受了[1,1,2,3,3-(2)H(5)]甘油的首剂量-持续输注以及[1-(13)C]棕榈酸的持续输注，以测量甘油和血浆FA动力学。在第1天（D1），参与者按照随机顺序在三种不同条件之一进行研究：（1）在45%最大摄氧量（V(O2)peak）下运动90分钟之前、期间及之后3小时（E45）；（2）在65% V(O2)peak下运动60分钟之前、期间及之后3小时（E65）；（3）在时间匹配的久坐对照试验（C）中。对于每种条件，次日早晨（D2）也通过间接测热法对参与者进行了研究。甘油的出现率（Ra）（Ra(GL)）在男性和女性运动期间均高于C组（P < 0.05），男性中E45组的Ra(GL)高于E65组（P < 0.05），而女性中不同运动强度之间无差异。在运动后恢复的3小时内，男性的Ra(GL)仍显著升高（P < 0.05），但女性则不然。FA的Ra（Ra(FA)）在男性和女性运动期间均增加，且E45组高于E65组（P < 0.05），在运动后恢复的3小时内两性均保持升高（P < 0.05），但男性的相对增加幅度大于女性（P < 0.05）。血浆FA氧化（Rox）在运动期间增加，各强度间无差异，在运动后恢复的3小时内两性均保持升高（P < 0.05）。总脂质氧化（Lox）在两性中均升高（P < 0.05），但在D1运动后恢复的3小时内男性升高更明显（P < 0.05），且在D2时男性仍保持升高（P < 0.05），女性则不然。由于各运动组间运动能量消耗（EEE）相匹配，因此男性和女性在运动后能量底物周转或氧化方面，E45组和E65组之间无差异。我们得出结论，运动对脂质代谢的影响会持续到恢复阶段，但女性在运动期间更多依赖脂质，而在恢复期间，男性的脂质代谢增强程度更大。
