Milanick M A, Frame M D
University of Missouri, Department of Physiology, School of Medicine, Columbia.
Ann N Y Acad Sci. 1991;639:604-15. doi: 10.1111/j.1749-6632.1991.tb17358.x.
The kinetic equation that best describes the intracellular Na dependence of Ca influx into ferret red cells is sequential; whether this implies that there is a conformation of the protein that has both Na and Ca ions bound remains to be determined. Cd and Mn substitute very well for Ca on the exchanger in ferret red cells; this suggests that the Ca-binding site does not contain an important thiol and that the one of the Na steps may be rate limiting.
最能描述雪貂红细胞内钙离子流入对细胞内钠离子依赖性的动力学方程是顺序性的；这是否意味着存在一种同时结合了钠离子和钙离子的蛋白质构象仍有待确定。镉和锰在雪貂红细胞的离子交换器上能很好地替代钙离子；这表明钙离子结合位点不包含重要的硫醇基团，并且钠离子转运步骤之一可能是限速步骤。
