Lindblad P, Bergman B, Nierzwicki-Bauer S A
Department of Physiological Botany, University of Uppsala, Sweden.
Appl Environ Microbiol. 1991 Dec;57(12):3637-40. doi: 10.1128/aem.57.12.3637-3640.1991.
In situ immunogold labeling and transmission electron microscopy were used to detect nitrogenase in bacteria (bactobionts) symbiotically associated with leaf cavities of Azolla caroliniana and Azolla filiculoides. In A. caroliniana, the Fe protein of the nitrogenase complex was detected in a subset of the distinct bactobiont types present in leaf cavities of all ages. Similar results were obtained for the bactobionts of A. filiculoides with antisera against both the Fe and MoFe subunits of nitrogenase.
采用原位免疫金标记和透射电子显微镜技术检测与卡罗来纳满江红和细叶满江红叶腔共生的细菌(共生菌)中的固氮酶。在卡罗来纳满江红中,在所有年龄段叶腔中存在的不同共生菌类型的一个子集中检测到了固氮酶复合物的铁蛋白。用针对固氮酶铁亚基和钼铁亚基的抗血清对细叶满江红的共生菌进行检测,也得到了类似的结果。