Katano M, Kubota E, Yamamoto H, Nakamura M, Matsuo T, Hisatsugu T, Katsuki T, Koga H, Ikezaki K, Tabuchi K
Department of Surgery, Hospital Laboratory, Saga Medical School, Japan.
Biotherapy. 1991;3(4):373-9. doi: 10.1007/BF02221331.
When peripheral blood mononuclear cells (PBMC) were incubated with a streptococcal preparation, OK-432, for 24 h, PBMC acquired cytolytic activity against cultured and fresh human tumor cells. Such PBMC were called OK-432-activated mononuclear cells (OK-MC). OK-MC produce several kinds of cytokines such as interferon alpha (IFN alpha), IFN gamma, and tumor growth inhibitory factor (TGIF) both in vitro and in vivo. OK-MC-produced cytokines also inhibited the growth of cultured and fresh human tumor cells. The growth inhibition was examined by human tumor clonogenic assay using a double-layer agar technique. The results indicate that two pathways of anti-tumor activity are induced in OK-MC, i.e., cell-mediated and cytokine-mediated.
当外周血单个核细胞（PBMC）与链球菌制剂OK-432孵育24小时后，PBMC获得了针对培养的和新鲜的人肿瘤细胞的细胞溶解活性。这种PBMC被称为OK-432激活的单个核细胞（OK-MC）。OK-MC在体外和体内均产生多种细胞因子，如α干扰素（IFNα）、γ干扰素和肿瘤生长抑制因子（TGIF）。OK-MC产生的细胞因子也抑制培养的和新鲜的人肿瘤细胞的生长。采用双层琼脂技术通过人肿瘤克隆形成试验检测生长抑制情况。结果表明，OK-MC诱导了两条抗肿瘤活性途径，即细胞介导的和细胞因子介导的。
