Katano M, Mizoguchi T, Yamamoto H, Nakamura M, Matsuo T, Hisatugu T, Kisu T, Yamaoka K, Tokunaga O
Department of Surgery, Saga Medical School, Japan.
Jpn J Surg. 1990 Jan;20(1):76-82. doi: 10.1007/BF02470717.
The production of a tumor growth inhibitory factor (TGIF) was induced in human peripheral blood mononuclear cells (PBMC) by a streptococcal preparation, OK-432, in vitro. The antitumor effect of locally injecting PBMC treated with OK-432 into the tumor site was studied. PBMC were collected from patients with gastric cancer 5 to 12 days before their operation, and cultured with OK-432 for 24 hr in vitro. After the culture, the PBMC were washed thoroughly to eliminate the OK-432. The washed PBMC went on producing TGIF for more than 72 hr in vitro in the absence of OK-432. A small number of TGIF-producing PBMC, approximately 10(7) cells, were injected around the lesion under endoscopic observation. A remarkable antitumor effect was observed in 2 out of 10 cases of resectable gastric cancer. Histological examinations indicated that the antitumor effect is due to antitumor cytokines such as TGIF produced by PBMC rather than to the OK-432-activated PBMC themselves.
在体外，链球菌制剂OK-432可诱导人外周血单个核细胞（PBMC）产生肿瘤生长抑制因子（TGIF）。研究了将经OK-432处理的PBMC局部注射到肿瘤部位的抗肿瘤效果。在胃癌患者手术前5至12天采集PBMC，并在体外与OK-432培养24小时。培养后，将PBMC彻底洗涤以去除OK-432。洗涤后的PBMC在无OK-432的情况下在体外持续产生TGIF超过72小时。在内镜观察下，将少量产生TGIF的PBMC（约10⁷个细胞）注射到病变周围。在10例可切除胃癌病例中，有2例观察到显著的抗肿瘤效果。组织学检查表明，抗肿瘤效果是由于PBMC产生的诸如TGIF等抗肿瘤细胞因子，而非OK-432激活的PBMC本身。
