Vol'pe E A
Biull Eksp Biol Med. 1991 Aug;112(8):192-5.
The new cell variants were selected in vitro from the low-malignant Syrian hamster embryo cells (STHE strain) spontaneously transformed in vitro. Ten cycles of in vitro co-cultivation of the parental STHE cells with the normal Syrian hamsters peritoneal exudate cells (PEC) (resident and LPS-activated PEC) used as a selecting agents (PERr, PECa) were performed. The susceptibilities of thus selected STHE cell variants as well as parental STHE cells to macrophage cytotoxicity and H2O2 dagame were tested with 3H-TdR assays. It has been demonstrated that all five STHE cell variants selected in vitro with PECa, in contrast to the parental STHE cells and three STHE cell variants selected with the use of PECr, were significantly more resistant to H2O2-mediated cytotoxicity. The increased resistance was acquired already after the 1-st cycle of the selection procedure. In contrast, only one STHE cell variant selected with PECr appeared to be semiresistant (demonstrated in 4 out of 6 experiments) to H2O2 damage after 10th cycle of in vitro co-cultivation.
新的细胞变体是从体外自发转化的低恶性叙利亚仓鼠胚胎细胞（STHE株）中体外筛选出来的。将亲代STHE细胞与正常叙利亚仓鼠腹腔渗出细胞（PEC）（驻留和LPS激活的PEC）作为选择剂（PERr、PECa）进行了10轮体外共培养。用3H-TdR试验检测了如此筛选出的STHE细胞变体以及亲代STHE细胞对巨噬细胞细胞毒性和H2O2损伤的敏感性。结果表明，与亲代STHE细胞和使用PECr筛选出的3个STHE细胞变体相比，用PECa体外筛选出的所有5个STHE细胞变体对H2O2介导的细胞毒性具有显著更高的抗性。在选择程序的第1个周期后就已经获得了增强的抗性。相比之下，在体外共培养第10个周期后，用PECr筛选出的只有1个STHE细胞变体对H2O2损伤表现出半抗性（在6个实验中的4个中得到证实）。
