酵母乌头酸酶的穆斯堡尔效应和电子顺磁共振研究

Mössbauer effect and electron paramagnetic resonance studies on yeast aconitase.

作者信息

Suzuki Y, Maeda Y, Sakai H, Fujimoto S, Morita Y

出版信息

J Biochem. 1975 Sep;78(3):555-60. doi: 10.1093/oxfordjournals.jbchem.a130940.

DOI:10.1093/oxfordjournals.jbchem.a130940

Abstract

Mössbauer effect and electron paramagnetic resonance (EPR) were measured for yeast aconitase [EC 4.2.1.3] purified from the cells of Candida lipolytica (ATCC 200114). Mössbauer spectra suggested that yeast aconitase nostly contained two high-spin Fe(III) ions in an antiferromagnetically coupled binuclear complex that resembled oxidized 2 Fe ferredoxins, together with a small amount of high-spin Fe(II). EPR spectra recorded no signal at 77degreesK, but showed a slightly asymmetric signal centered at g=2.0 at 4.2degreesK, presumably due to the small amount of Fe(II) Fe(III) pairs.

摘要

对从解脂耶氏酵母（ATCC 200114）细胞中纯化得到的酵母乌头酸酶[EC 4.2.1.3]进行了穆斯堡尔效应和电子顺磁共振（EPR）测量。穆斯堡尔光谱表明，酵母乌头酸酶主要含有两个处于反铁磁耦合双核络合物中的高自旋Fe(III)离子，该双核络合物类似于氧化型2Fe铁氧化还原蛋白，还含有少量高自旋Fe(II)。EPR光谱在77K时未记录到信号，但在4.2K时显示出一个以g=2.0为中心的略微不对称信号，可能是由于少量的Fe(II)-Fe(III)对所致。