对氯苯氧异丁酸乙酯对大鼠肝脏碳水化合物和脂肪酸代谢的影响。

Effects of ethyl p-chlorophenoxyisobutyrate on carbohydrate and fatty acid metabolism in rat liver.

Miyazawa S, Sakurai T, Imura M, Hashimoto T

J Biochem. 1975 Dec;78(6):1171-6. doi: 10.1093/oxfordjournals.jbchem.a131013.

Abstract

Rats were fed on a diet containing ethyl p-chlorophenoxyisobutyrate (0.3%, w/w) for 14 days. 2. The alterations of contents of intermediates in the liver indicate that gluconeogenesis is inhibited at the reaction(s) between 3-phosphoglycerate and fructose 1,6-diphosphate. The [nad+]/([nadh] ratios in cytoplasm and mitochondria were increased about 3- and 4-fold, respectively. Marked increases in the contents of CoA and its thioesters were found. 3. Hepatic fatty acid synthesis increased about 3-fold. There was no evidence of inhibition of the acetyl-CoA carboxylase [EC 6.4.1.2] reaction by the drug.

摘要

给大鼠喂食含对氯苯氧异丁酸乙酯（0.3%，w/w）的饲料14天。2. 肝脏中中间产物含量的变化表明，糖异生在3-磷酸甘油酸和1,6-二磷酸果糖之间的反应处受到抑制。细胞质和线粒体中的[NAD⁺]/[NADH]比值分别增加了约3倍和4倍。发现辅酶A及其硫酯的含量显著增加。3. 肝脏脂肪酸合成增加了约3倍。没有证据表明该药物抑制乙酰辅酶A羧化酶[EC 6.4.1.2]反应。