Liou S Y, Albers H E
Department of Biology and Psychology George State University, Atlanta 30303.
Brain Res Bull. 1991 Dec;27(6):825-8. doi: 10.1016/0361-9230(91)90216-7.
The effect of neuropeptide Y (NPY) on the spontaneous discharge of neurons within the hamster (Mesocricetus auratus) suprachiasmatic nucleus (SCN) (N = 83) was determined using the hypothalamic slice preparation. The discharge of neurons within the ventral SCN recorded during the day was either excited (6/42), immediately inhibited (17/42), or transiently excited and then inhibited (10/42) by NPY 10(-7). During the night, NPY produced excitatory effects in 2/23 neurons, inhibition in 7/23 and excitation followed by inhibition in 4/23. A higher percentage of neurons was found to be unresponsive to NPY during the night than during the day. This difference approached but did not reach statistical significance. In the 18 neurons recorded within the dorsal SCN, NPY had little effect on spontaneous discharge during the day or night. These data indicate that bath application of NPY predominately inhibits the spontaneous discharge of SCN neurons recorded in the hypothalamic slice preparation.
利用下丘脑切片标本，测定了神经肽Y（NPY）对仓鼠（金黄地鼠）视交叉上核（SCN）（N = 83）内神经元自发放电的影响。白天记录的腹侧SCN内神经元的放电，10⁻⁷的NPY使其兴奋（6/42）、立即抑制（17/42）或先短暂兴奋然后抑制（10/42）。夜间，NPY对2/23的神经元产生兴奋作用，对7/23的神经元产生抑制作用，对4/23的神经元先兴奋后抑制。发现夜间对NPY无反应的神经元百分比高于白天。这种差异接近但未达到统计学显著性。在背侧SCN记录的18个神经元中，NPY在白天或夜间对自发放电几乎没有影响。这些数据表明，浴用NPY主要抑制下丘脑切片标本中记录的SCN神经元的自发放电。
