Zhu Q F
Henan Medical University, Zhengzhou.
Zhonghua Zhong Liu Za Zhi. 1991 May;13(3):171-3.
The frequencies of micronuclei and apoptosis in the colon crypt cells of mice treated with colon carcinogen were studied. Two strains of mice, inbred C57BL and close colony Kun-ming mice were used in this experiment. The mice were killed at 24 hr after intraperitoneal injection of dimethylhydrazine (DMH) at doses of 10, 20 and 40 mg/kg body weight. The results indicated that the frequencies of micronuclei and apoptosis in the colon crypt cells of both C57BL and Kun-ming mice were positively correlated quantitatively with the doses of DMH. Our results are similar to that of Heddle obtained in C57BL mice. We propose that the assay of micronuclei and apoptosis in mouse colon crypt cells might be a rapid and sensitive test and useful as a screening method for potential colon carcinogens. Kun-ming mice may be used as the test animal for detecting nuclear anomalies in this assay in lieu of C57BL mice.
研究了用结肠致癌物处理的小鼠结肠隐窝细胞中微核和凋亡的频率。本实验使用了两种品系的小鼠，近交系C57BL小鼠和封闭群昆明小鼠。在腹腔注射剂量为10、20和40mg/kg体重的二甲基肼（DMH）24小时后处死小鼠。结果表明，C57BL小鼠和昆明小鼠结肠隐窝细胞中微核和凋亡的频率与DMH剂量呈正相关。我们的结果与Heddle在C57BL小鼠中获得的结果相似。我们提出，小鼠结肠隐窝细胞中微核和凋亡的检测可能是一种快速、灵敏的检测方法，可作为潜在结肠致癌物的筛选方法。在该检测中，昆明小鼠可代替C57BL小鼠作为检测核异常的实验动物。
