Blakey D H, Duncan A M, Wargovich M J, Goldberg M T, Bruce W R, Heddle J A
Cancer Res. 1985 Jan;45(1):242-9.
Colon carcinogens produce a variety of nuclear anomalies including pyknotic, karyorrhectic, and micronucleated cells in the colonic epithelium within a few hr. Two model carcinogens, 1,2-dimethylhydrazine and gamma-rays, have been used to determine appropriate techniques, conditions, and scoring criteria for detecting such nuclear anomalies most efficiently. The results show that a rapid and sensitive assay for nuclear anomalies can be conducted with a variety of preparation techniques. We anticipate that the assay will be useful as a screen for potential colon carcinogens in the diet or elsewhere. The assay as recommended by us requires at least five animals per group and takes about 1 hr to analyze. A single sample at 6 or 24 hr after treatment should detect most carcinogens.
结肠致癌物在数小时内会在结肠上皮细胞中产生多种核异常，包括固缩、核溶解和微核化细胞。两种模型致癌物，即1,2 - 二甲基肼和γ射线，已被用于确定最有效地检测此类核异常的合适技术、条件和评分标准。结果表明，使用多种制备技术可以进行快速且灵敏的核异常检测。我们预计该检测方法将有助于筛选饮食或其他来源中的潜在结肠致癌物。我们推荐的检测方法每组至少需要五只动物，分析大约需要1小时。处理后6小时或24小时的单个样本应能检测出大多数致癌物。
