Mujat Mircea, Park B Hyle, Cense Barry, Chen Teresa C, de Boer Johannes F
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 40 Blossom Street, BAR 704, Boston, Massachusetts 02114, USA.
J Biomed Opt. 2007 Jul-Aug;12(4):041205. doi: 10.1117/1.2764460.
Accurate wavelength assignment of each spectral element for spectral-domain optical coherence tomography (SD-OCT) and optical frequency domain imaging (OFDI) is required for proper construction of biological tissue cross-sectional images. This becomes more critical for functional extensions of these techniques, especially in polarization-sensitive optical coherence tomography (PS-OCT), where incorrect wavelength assignment between the two orthogonal polarization channels leads to polarization artifacts. We present an autocalibration method for wavelength assignment that does not require separate calibration measurements and that can be applied directly on actual data. Removal of the birefringence artifact is demonstrated in a PS-OCT system with picometer accuracy in the relative wavelength assignment, resulting in a residual phase error of 0.25 deg/100 microm. We also demonstrate, for the first time, a quantitative birefringence map of an in vivo human retinal nerve fiber layer.
对于光谱域光学相干断层扫描（SD-OCT）和光学频域成像（OFDI），要正确构建生物组织横截面图像，需要为每个光谱元素精确分配波长。对于这些技术的功能扩展而言，这一点变得更为关键，特别是在偏振敏感光学相干断层扫描（PS-OCT）中，两个正交偏振通道之间的波长分配错误会导致偏振伪像。我们提出了一种用于波长分配的自动校准方法，该方法不需要单独的校准测量，并且可以直接应用于实际数据。在一个PS-OCT系统中，展示了以皮米精度进行相对波长分配来消除双折射伪像，从而在100微米的距离上产生0.25度的残余相位误差。我们还首次展示了人体视网膜神经纤维层的定量双折射图。
