Kim Gwangseong, Huang Sheng-Wen, Day Kathleen C, O'Donnell Matthew, Agayan Rodney R, Day Mark A, Kopelman Raoul, Ashkenazi Shai
University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109, USA.
J Biomed Opt. 2007 Jul-Aug;12(4):044020. doi: 10.1117/1.2771530.
Nanoparticles 100 nm in diameter containing indocyanine green (ICG) have been developed as a contrast agent for photoacoustic (PA) imaging based on (photonic explorers for biomedical use by biologically localized embedding PEBBLE) technology using organically modified silicate (ormosil) as a matrix. ICG is an FDA-approved dye with strong optical absorption in the near-infrared (NIR) region, where light can penetrate deepest into biological tissue. A photoacoustic imaging system was used to study image contrast as a function of PEBBLE concentration in phantom objects. ICG-embedded ormosil PEBBLEs showed improved stability in aqueous solution compared with free ICG dye. The particles were conjugated with HER-2 antibody for breast cancer and prostate cancer cell targeting. Initial in vitro characterization shows high contrast and high efficiency for binding to prostate cancer cells. ICG can also be used as a photosensitizer (generating toxic oxygen by illumination) for photodynamic therapy. We have measured the photosensitization capability of ICG-embedded ormosil nanoparticles. This feature can be utilized to combine detection and therapeutic functions in a single agent.
直径为100纳米、含有吲哚菁绿（ICG）的纳米颗粒已被开发为一种基于（通过生物定位嵌入实现生物医学用途的光子探测器，即PEBBLE）技术、以有机改性硅酸盐（ormosil）为基质的光声（PA）成像造影剂。ICG是一种经美国食品药品监督管理局（FDA）批准的染料，在近红外（NIR）区域具有很强的光吸收能力，而在该区域光能够穿透生物组织最深。使用光声成像系统研究了幻影物体中作为PEBBLE浓度函数的图像对比度。与游离ICG染料相比，嵌入ICG的ormosil PEBBLE在水溶液中表现出更高的稳定性。这些颗粒与用于乳腺癌和前列腺癌细胞靶向的HER-2抗体偶联。初步的体外表征显示其与前列腺癌细胞结合具有高对比度和高效率。ICG还可以用作光动力疗法的光敏剂（通过光照产生有毒氧气）。我们已经测量了嵌入ICG的ormosil纳米颗粒的光敏能力。这一特性可用于在单一制剂中结合检测和治疗功能。
