Qian Haili, Yu Jing, Li Yunfeng, Wang Haijuan, Song Chongwen, Zhang Xueyan, Liang Xiao, Fu Ming, Lin Chen
State Key Laboratory of Molecular Oncology, Cancer Institute/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan Nanli, Chao Yang District, Beijing 100021, People's Republic of China.
Biol Cell. 2007 Oct;99(10):573-81. doi: 10.1042/bc20060130.
MTA1 (metastasis-associated gene 1) has been reported to be overexpressed in cancers with high potential to metastasize. Studies of the molecular mechanisms revealed that MTA1 plays an important role in the process of metastasis of many types of cancer. However, the role of MTA1 in melanoma development is unclear.
We have investigated the therapeutic value of MTA1 in the B16F10 melanoma cell line with the C57BL/6 mouse model. Studies in vitro showed that MTA1 promoted the metastatic ability of B16F10 cancer cells. MTA1 down-regulation by RNA interference greatly reversed the malignant phenotypes of cancer cells. Immunohistochemical staining of MTA1 in human melanoma samples confirmed the up-regulation of MTA1 in the process of carcinogenesis. Studies in vivo confirmed down-regulation of MTA1 suppressed the growth and experimental metastasis of B16F10 melanoma cells.
MTA1 plays an important role in melanoma development and metastasis. It has a promising potential as a target for in cancer gene therapy or chemotherapy.
据报道，转移相关基因1（MTA1）在具有高转移潜能的癌症中过表达。分子机制研究表明，MTA1在多种癌症的转移过程中起重要作用。然而，MTA1在黑色素瘤发展中的作用尚不清楚。
我们用C57BL/6小鼠模型研究了MTA1在B16F10黑色素瘤细胞系中的治疗价值。体外研究表明，MTA1促进了B16F10癌细胞的转移能力。RNA干扰下调MTA1可极大地逆转癌细胞的恶性表型。人黑色素瘤样本中MTA1的免疫组化染色证实了MTA1在致癌过程中的上调。体内研究证实，下调MTA1可抑制B16F10黑色素瘤细胞的生长和实验性转移。
MTA1在黑色素瘤的发展和转移中起重要作用。作为癌症基因治疗或化疗的靶点，它具有广阔的应用前景。
