Vallotton Pascal, Lagerstrom Ryan, Sun Changming, Buckley Michael, Wang Dadong, De Silva Melanie, Tan Seong-Seng, Gunnersen Jenny M
CSIRO, Mathematical and Information Sciences, North Ryde, NSW, Australia.
Cytometry A. 2007 Oct;71(10):889-95. doi: 10.1002/cyto.a.20462.
Manual neuron tracing is a very labor-intensive task. In the drug screening context, the sheer number of images to process means that this approach is unrealistic. Moreover, the lack of reproducibility, objectivity, and auditing capability of manual tracing is limiting even in the context of smaller studies. We have developed fast, sensitive, and reliable algorithms for the purpose of detecting and analyzing neurites in cell cultures, and we have integrated them in software called HCA-Vision, suitable for the research environment. We validate the software on images of cortical neurons by comparing results obtained using HCA-Vision with those obtained using an established semi-automated tracing solution (NeuronJ). The effect of the Sez-6 deletion was characterized in detail. Sez-6 null neurons exhibited a significant increase in neurite branching, although the neurite field area was unchanged due to a reduction in mean branch length. HCA-Vision delivered considerable speed benefits and reliable traces.
手动神经元追踪是一项劳动强度极大的任务。在药物筛选的背景下，需要处理的图像数量之多意味着这种方法不切实际。此外，即使在较小规模研究的背景下，手动追踪缺乏可重复性、客观性和审核能力也具有局限性。我们开发了快速、灵敏且可靠的算法，用于检测和分析细胞培养中的神经突，并将其集成到适用于研究环境的名为HCA-Vision的软件中。我们通过将使用HCA-Vision获得的结果与使用既定的半自动追踪解决方案（NeuronJ）获得的结果进行比较，在皮质神经元图像上验证了该软件。详细表征了Sez-6缺失的影响。Sez-6基因敲除的神经元神经突分支显著增加，尽管由于平均分支长度的减少，神经突场面积没有变化。HCA-Vision带来了显著的速度优势和可靠的追踪结果。
